结直肠癌患者外周血与肿瘤组织中TAZ表达的关系及临床意义研究

摘要

目的 探讨转录共激活因子(TAZ)在结直肠癌患者外周血与肿瘤组织中表达,分析其关系及临床意义.方法 选取经病理确诊为结直肠癌并行根治性手术患者85例为手术组,手术后确诊复发的患者35例作为复发组,同时选取年龄性别相匹配的健康人50例为对照组.取结直肠癌患者肿瘤组织及正常黏膜组织石蜡标本,采用免疫组织化学染色检测TAZ表达,酶联免疫吸附试验(ELISA)检测各组外周血中TAZ的水平.结果 手术组患者术前外周血中TAZ水平明显高于对照组(P＜005),手术后TAZ水平较对照组无明显变化(P＞0.05),复发组患者外周血TAZ水平明显高于手术组术前(P＜0.05).手术组患者肿瘤组织中TAZ表达明显高于正常黏膜组织(P＜0.05),复发组患者肿瘤组织中TAZ表达高于手术组(P＜0.05).手术组和复发组患者肿瘤组织与外周血TAZ水平呈正相关性(P＜0.05).结论 监测TAZ水平对及时了解肿瘤复发有明显的参考价值.%Objective To investigate the expression of TAZ in peripheral blood and tumor tissue of the patients with colorectal cancer and to analyze their relationship and clinical significance.Methods Eighty-five cases of pathologically verified colorectal cancer treated by radical operation were selected as the operation group,and 35 cases with postoperative relapse served as the recurrent group.Meanwhile 50 age and sex-matched healthy persons were selected as the control group.The immunohistochemical(SP)technique was adopted to detect the expression of TAZ in tumor tissues and expression of TAZ in peripheral blood was tested with enzyme linked immunosorbent assay(ELISA).Results The level of preoperative TAZ in peripheral blood of the operation group was significantly higher than that of the control group(P＜0.05).The level of peripheral blood TAZ after operation in the operation group had no significant change compared with the control group (P＞0.05).The level of peripheral blood TAZ in the recurrent group was significantly higher than preoperative level of operation group(P＜0.05).The expression of tumor tissue TAZ was significantly higher than that of normal mucosal tissue(P＜0.05);the expression of tumor tissue TAZ in the recurrent group was higher than that in the operation group(P＜0.05).The tumor tissue TAZ level in the operation group and recurrent group had obvious correlation with peripheral blood TAZ level(P＜0.05).Conclusion Monitoring the TAZ level has obvious reference value for predicting colorectal cancer staging and timely understanding tumor recurrence.