小青龙汤对哮喘模型小鼠气道上皮细胞表达TSLP的影响研究

摘要

目的 探讨小青龙汤对哮喘模型小鼠气道上皮细胞表达胸腺基质淋巴细胞生成素(TSLP)的影响.方法 将30只健康雌性BALB/C小鼠分为空白对照组、哮喘模型组、小青龙汤组,每组10只.各组采取相应干预措施后取支气管、肺组织,免疫荧光检测支气管TSLP的表达水平,qRT-PCR和Western blot分别检测肺组织TSLP mRNA和蛋白表达水平.结果 小鼠支气管上皮细胞膜上TSLP荧光染色在哮喘模型组呈强阳性,小青龙汤组呈弱阳性,空白对照组呈阴性.小青龙汤组与空白对照组小鼠肺组织 TSLP mRNA表达水平较哮喘模型组降低(P<0.05),小青龙汤组略高于空白对照组,但组间差异无统计学意义(P>0.05).小青龙汤组与空白对照组小鼠肺组织TSLP蛋白表达水平均低于哮喘模型组(P<0.05),小青龙汤组较空白对照组略高,但组间差异无统计学意义(P>0.05).结论 小青龙汤对哮喘模型小鼠气道上皮细胞表达TSLP有明显抑制作用.%Objective To study the effect of Xiaoqinglong Decoction on the expression of TSLP in airway epithelial cells of asthmatic model mice.Methods Thirty healthy female BALB/C mice were divided into the blank control group,asthma model group and Xiaoqinglong Decoction group,10 cases in each group.The each group adopted corresponding intervention measures. Then the bronchial and lung tissues were taken.The expression level of bronchial TSLP was detected by immunofluorescence,and the expression level of TSLP mRNA and protein in lung tissue was detected by qRT-PCR and Western Blot.Results The mouse bronchial epithelial cell membrane TSLP staining was strongly positive in the asthma model group,which was weakly positive in the Xiaoqinglong Decoction group and negative in the blank control group.Compared with the asthma model group,mice lung tissue TSLP mRNA expression level in the Xiaoqinglong Decoction group and blank control group was decreased(P<0.05),the Xiaoqin-glong Decoction group was slightly higher than the blank control group,but the inter-group difference was not statistically signifi-cant(P>0.05).The expression level of lung tissue TSLP protein in the Xiaoqinglong Decoction group and blank control group was lower than that in the asthma model group(P<0.05),the Xiaoqinglong Decoction group was slightly higher than the blank control group,but the inter-group difference was not statistically significant(P>0.05).Conclusion Xiaoqinglong decoction has obvious inhibiting effect on the expression of TSLP in airway epithelial cells of asthmatic model mice.