UPLC-MS/MS法测定烟草中12种常用农药残留量

摘要

In order to rapidly determine common pesticide residues in tobacco, a rapid method for determining twelve pesticides in tobacco using UPLC-MS/MS was developed. Samples were extracted by acetonitrile, cleaned up by dispersive solid-phase extraction and finally detected by UPLC-MS/MS. The results showed that the recoveries of twelve pesticides ranged from 62.1%-126.3%at the spiking levels of 20 µg/kg, 200 µg/kg and 500 µg/kg. The RSD ranged from 0.6%-14.8%, and the LOD was from 0.24 µg/kg to 4.24 µg/kg. For the superior sensitivity, targeted residues and shorter analysis time, this method was suitable for the rapid analysis of common pesticide residues in batches of tobacco samples.%为了实现烟草中常用农药残留量的快速筛查，建立了超高效液相色谱-串联质谱（UPLC-MS/MS）快速分析烟草中12种农药残留量的方法，烟叶样品采用乙腈提取、PSA净化和UPLC-MS/MS分析测定。12种农药在低、中、高（2、200、500µg/kg）3个加标水平的回收率范围为62.1%~126.3%，相对标准偏差（RSD）为0.6%~14.8%，检出限为0.24~4.24µg/kg。本方法具有针对性强，检测时间短和灵敏度高的特点，能够很好的应用于烟草中常用农药残留量的快速分析检测。