The leaf streak of rice is one of the seedborne and quarantine bacterial disease in China. The rice seeds must be strictly inspected to prevent the disease from spreading. The serological test is a rapid and accurate method of identifying seedborne bacterial plant pathogens. But the specificity and thus reliability of any serological method depend first on the specificity of the antiserum. Therefore, a high specificity of antiserum is always required for and has become a key in detecting the leaf streak pathogen in rice seeds. The present study was conducted on the method of improving the specificity. Two group of antigen, the yellow and white proteins, were extracted from the cells of Xanthornonas oryzae pv. oryzicola by the methods of stepwise precipitation with ammonium sulfate and differential centrifugation. Antiserum A and B against the yellow and white proteins were each produced in white rabbit immunized with the proteins respectively. The tittle of the two antiserum were the same(1: 6400).
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