白芍总苷对胶原性关节炎大鼠滑膜β抑制蛋白的影响与其抑制滑膜细胞增殖的关系

摘要

目的 探讨白芍总苷(TGP)对胶原性关节炎(CIA)大鼠滑膜β抑制蛋白1、2表达的影响与其抑制成纤维样滑膜细胞(FLS)增殖的关系.方法 采用CCⅡ诱导大鼠CIA模型,TGP(25、50、100 mg·kg-1·d-1)、雷公藤多苷(GTW)(40 mg·kg-1·d-1)灌胃给药(d 14~28),进行大鼠多发性关节炎指数评分;MTT法检测FLS的增殖;Western blot法检测不同剂量TGP体内给药对β抑制蛋白1、2表达的影响.结果 TGP(25、50、100 mg·kg-1,ig,d14~28)可明显减轻CIA大鼠多发性关节炎指数,抑制FLS增殖反应;TGP可下调β抑制蛋白2的表达;TGP对β抑制蛋白2表达的影响与其抑制FLS增殖作用呈高度相关.结论 TGP可抑制FLS的增殖,其机制可能与其下调β抑制蛋白2的表达有关.%Aim To elucidate the correlation between effects of total glucosides of paeony (TGP) on expression of β-αrrestins and inhibition on synoviocyte proliferation from CIA rats. Methods CIA model in rats was induced by injection of chicken type II collagen (CCII) emulsion. TGP (25, 50, 100 mg · kg-1 · d-1 ) was adminisered intragastricly to CIA rats during d 14 ~28. Synoviocyte proliferation was determined by MTT assay. The expression of P-αrrestins in synovio- cytes from CIA rats was measured by Western blot. Results TGP (25,50, 100 mg · kg-1, ig, d 14 ~28) could inhibit synoviocyte proliferation and reduce the expression of P-αrrestin 2 greatly in synovial tissue of CIA rats. The effects of TGP on the expression of β-αrrestin 2 had a positive correlation with the inhibition of TGP on synoviocyte proliferation. Conclusion TGP can inhibit synoviocyte proliferation of CIA rats , which may be related to downregulating the expression of β-αrrestin 2.