新藤黄酸诱导HepG2细胞凋亡与Bax及Bcl-2的关系

摘要

Aim To explore the correlation betweendifferent indicators of neurological function in miceafter focal cerebral ischemia model( MCAO ). Methods Suture method was used to produce permanent focal cerebral ischemia model. Different neurological function indicators were selected and percentage of brain infarction was measured to evaluate the damage degree of model. Correlation coefficient between indicators were obtained. Puerarin and Edaravone were used to validate the rationality of chosen indicators. Results There was significant correlation between neurological behavioral and other neurological function indicators af-ter operation. Cerebral infarction size percentage was significantly correlated with neurological comprehensive function and inclined test except corner test. Puerarin and Edaravone significantly inhibited the cerebral ischemia injury in mice. Conclusion The above indicators can be used to assess the effects of drugs on cerebral ischemia in mice.%@@ 中药藤黄（gamboge）系藤黄科植物藤黄树（Garcinia hanburyi Hook．F．G）所分泌的干燥树脂,近年来,藤黄的抗肿瘤作用受到高度关注.已有研究证实,新藤黄酸（neogambogic acid, NGA）为藤黄抗肿瘤作用的主要有效成分之一［1-3］.肝细胞癌（hepatocellular carcinoma,HCC）是世界上最常见的恶性肿瘤之一［4］,同时也是第三大引起患者死亡的肿瘤［5］.为此,本实验探讨了新藤黄酸诱导肝细胞癌HepG2细胞凋亡的作用机制,旨在为进一步开发新藤黄酸提供实验依据.