灵芝总三萜与紫杉醇或与顺铂在对人源肿瘤细胞的细胞毒性中的相互作用

摘要

目的:灵芝三萜类化合物是常被用于辅助性肿瘤治疗的灵芝中具有细胞毒性作用的主要成分.本研究考察灵芝总三萜和化疗药物之间的相互作用.方法:应用了两种肿瘤细胞株即人宫颈瘤HeLa细胞株和人非小细胞肺癌A549细胞株.检测了将灵芝总三萜与紫杉醇或顺铂合用时对细胞的毒性作用,并用Chou and Talalay合用指数法判断合用的相互作用类型.结果:灵芝总三萜和紫杉醇的合用在HeLa细胞中表现为协同作用而在A549细胞中表现为加合作用.灵芝总三萜与顺铂的合用效果则依赖于该两种药物的作用强度,即当其中一种药物的作用强于另外一种时表现为拮抗作用,而当两种药物的作用相似时表现为协同作用.结论:灵芝总三萜可以促进某些化疗药物的作用.%AIM: Ganoderma triterpenes (GTS) are the main components with cytotoxicity in Ganoderma lucidum,a popularly used traditional Chinese Medicine (TCM) for complementary cancer therapy.The present study was designed to investigate the possible interaction between Ganoderma triterpenes and chemotherapeutics.METHODS: Cultures of human cervical carcinoma HeLa cells and human non-small lung carcinoma A549 cells were used.The cytotoxic effects-of GTS in combination with docetaxel (TXT) or eisplatin (CDDP) on cells were examined and the combination effects were analyzed by the Cbou and Talalay combination index method.RESULTS: The combinations targeting GTS with TXT resulted in a synergistic interaction in HeLa cells and an additive interaction in A549 cells.While,in both HeLa and A549 cells,the interactions between GTS and CDDP depended on the relative potency of their cytotoxicity.The interaction was antagonism when the cytotoxicity of GTS or CDDP was stronger than that of the other and was synergism when the cytotoxicity of the two medicines was similar.CONCLUSION: GTS could enhance the cytotoxieity of some kinds of chemotherapeutics but not all ehemotherapeutics against human carcinoma cells.