目的探讨并评估体外扩增的自体脂肪干细胞介导自体脂肪移植的治疗效果。 方法实验组50例患者经过自体脂肪干细胞分离、培养、体外扩增后，介导自体脂肪颗粒注射移植，充填面部塑形、隆胸，随访6~24个月。手术分为两期，第一期：取患者自体脂肪颗粒细胞15~20 ml，进行体外分离、培养、扩增，扩增至第3代，进行细胞免疫表型、分化潜能、遗传稳定性鉴定。第二期：20~25 d 后，抽取一定量的患者自体脂肪颗粒混合第5~6代自体脂肪干细胞移植，用于鼻部、颏部、额部及颞部等面部充填塑形和隆胸。对照组10例患者，单纯自体脂肪移植。 结果第3~6代自体脂肪干细胞具有典型的间充质干细胞特征，并保持了遗传稳定性。脂肪移植6~24个月后，回访无明显脂肪吸收现象，塑形效果良好。 结论自体脂肪干细胞介导的自体脂肪移植术具有微创、移植脂肪成活率高、成形效果好等优点，是具有发展前途的、值得推荐的再生医学技术。%Objective To investigate and evaluate the effect of the adipose derived stem cells in autologous fat transplantation. Methods The whole operation was separated into two phases. At first, 15 - 20 ml adipose tissue was isolated, cultured and expanded to obtain human adipose-derived stem cells (hADSCs). When cultured to passage 3-6, the plastic adherentstem cells were verified by immunophenotype, differentiation and genetic stability assays before transplantation. Secondly, the adipose tissue was harvested by standard sterile liposuction and combined with hADSCs for cosmetic transplantation. Results hADSCs at the passage 3-6 exhibited typical characteristics and maintained genetic stability of mesenchymal stem cells. There exited no apparent adipose absorption and a good shaping effect in the following 6-24 months. Conclusion Autologous adipose transplantation mediated by hADSCs to be used as face cosmetic supplyment and breast augmentation is a practical and efficacious method.
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