Vimentin modulates apoptosis and inflammatory cytokine release by a human monocytic cell line (THP-1) in response to lipopolysaccharides in vitro

摘要

Background:It has recently been recognized that serum vimentin is elevated in infectious diseases,and that vimentin plays a role in regulating neutrophils and macrophages associated inflammation.However,the mechanisms are unclear.This study was designed to explore the role of vimentin in regulating monocyte survival or apoptosis as well as inflammatory cytokine secretion in response to lipopolysaccharides (LPSs).Methods:A human monocytic leukemia cell line (THP-1) was transfected with vimentin-specific small interfering RNA (siRNA) or vimentin over-expressing plasmid.Apoptosis was assessed by TdT-mediated dUTP Nick-End Labeling (TUNEL) and DNA content assay.Immunoblotting was performed to detect apoptosis-associated proteins.Cytokines (interleukin [IL]-6,IL-10,and tumor necrosis factor α [TNF-α]) were measured by enzyme-linked immuno sorbent assay.Two-way analysis of variance followed by Student's t test was used to compare means between different groups.Results:Suppression of vimentin in THP-1 cells resulted in increased apoptotic response in the presence of LPS,while overexpression of vimentin could prevent the cells from apoptosis in response to LPS.LPS alone or suppression of vimentin resulted in significant up-regulation of caspase-3 (1.42 ± 0.20 of LPS alone and 1.68 ± 0.10 of vimentin suppression vs.control,t =5.21 and 10.28,respectively,P ＜ 0.05).In addition,pro-inflammatory cytokines (IL-6 and TNF-α) was significantly increased (IL-6:577.90 ± 159.90 pg/day/105 cells vs.283.80 ± 124.60 pg/day/105 cells of control,t=14.76,P ＜ 0.05;TNF-α:54.10 ± 5.80 vs.17.10 ± 0.10 pg/day/105 cells of control,t =6.71,P ＜ 0.05),while anti-inflammatory cytokine (IL-10) was significantly up-regulated in the THP-1 cells that over-expressed vimentin (140.9 ± 17.2 pg/day/105 cells vs.undetectable in control cells).Contusions:In summary,the vimentin may regulate innate immunity through modulating monocytes viability as well as inflammatory response in sepsis through shifting the balance of pro-inflammatory and anti-inflammatory cytokines.