Rho-associated coiled kinase inhibitor Y-27632 promotes neuronal-like differentiation of adult human adipose tissue-derived stem cells

摘要

Background Y-27632 is a specific inhibitor of Rho-associated coiled kinase (ROCK) and has been shown to promote the survival and induce the differentiation of a variety of cells types.However,the effects of Y-27632 on adult human adipose tissue-derived stem cells (ADSCs) are unclear.This study aimed to investigate the effects of Y-27632 on the neuronal-like differentiation of ADSCs.Methods ADSCs were isolated from women undergoing plastic surgery and cultured.ADSCs were treated with different doses of Y-27632 and observed morphological changes under microscope.The expression of nestin,neuron specific enolase (NSE) and microtubule-associated protein-2 (MAP-2) in ADSCs treated with Y-27632 was detected by immunocytochemistry and Western blotting analysis.Results Y-27632 had the potency to induce neuronal-like differentiation in ADSCs in a dose-dependent manner.Moreover,the differentiation induced by Y-27632 was recovered upon drug withdraw.ADSCs treated with Y-27632 expressed neuronal markers such as NSE,MAP-2 and nestin while untreated ADSCs did not express these markers.Conclusion Selective ROCK inhibitor Y-27632 could potentiate the neuronal-like differentiation of ADSCs,suggesting that Y-27632 could be utilized to induce the differentiation of ADSCs to neurons and facilitate the clinical application of ADSCs in tissue engineering.