ATP生物荧光技术应用于软式内镜消毒质量检测的可行性研究

摘要

Objective To explore the feasibility of applying ATP bioluminescence technology to disinfection quality monitoring of the flexible endoscope.Methods Totally 30 flexible endoscopes used repeatedly from October 2014 to March 2015 were randomly selected,and had the disinfection quality monitored by ATP bioluminescence technology and bacterial culture method respectively.Parallel comparison was carried out to evaluate the feasibility of applying ATP bioluminescence technology to disinfection quality monitoring of the flexible endoscope.Results ATP bioluminescence technology showed that the qualification rate of the disinfection was 93.3％,and bacterial culture method found it was 96.7％.The two methods proved the outer surface of the endoscope had the disinfection acceptable while the biopsy hole and intracavity not.There was no significant correlation between the two methods while high consistency between the detection results by the two methods.Conclusion ATP bioluminescence technology can be used for preliminary screening in field,instant and daily monitoring of the medical flexible endoscope,which assists bacterial culture method in disinfection quality monitoring of the flexible endoscope.%目的:研究腺嘌呤核苷三磷酸(ATP)生物荧光检测法应用于软式内镜消毒效果检测的可行性.方法:随机抽取2014年10月至2015年3月科室重复使用的软式内镜,应用ATP生物荧光检测法及细菌培养计量法2种不同的检测方法,对随机抽取的30例软式内镜消毒效果进行检测,收集分析数据并作平行比较.结果:软式内镜消毒质量ATP生物荧光检测结果合格率为93.3％,细菌培养计量法合格率为96.7％.软式内镜外表面2种方法检测值均在合格范围,活检孔端口和内镜管腔内污染相对较重;2种检测方法并不具有显著的相关性,但其检测结果具有高度一致性.结论:ATP生物荧光检测技术可作为一种快速方便的初筛方法,实现医用软式内镜现场、即时、日常监测,辅助细菌培养计量法监控软式内镜的消毒质量.