番茄XRN2基因的克隆、表达分析及遗传转化

摘要

为了研究番茄XRN2基因的功能,利用RT-PCR技术从番茄各组织混合cDNA中扩增了番茄XRN2基因全长编码序列,并对其进行了表达模式分析.结果表明该基因在番茄根部表达最强烈.通过对番茄叶片进行伤害诱导处理,发现番茄XRN2基因表达呈上升趋势.将该基因在组成型启动子CaMV35S驱动下定向构建至植物表达载体pCambia1301-35S,重组载体命名为pCambia1301-35S-XRN2和pCambia1301-35S-AsXRN2.通过农杆菌介导法转化获得转基因番茄,为进一步阐明XRN2基因在番茄中的功能奠定了基础.%To understand the function of XRN2 gene in tomato, the full length SIXRN2 cDNA was amplified by RT-PCR from tomato mix cDNAs. Expression analysis results showed that SlXRN2 expressed the highest in tomato roots. The expression of XRN2 gene could be up-regulated by wound stress on the leaves. Then SlXRN2 gene driven by the constitutive promoter CaMV35S was directed cloning into plant expression vector pCambia1301-35S,named as pCambia1301-35S-XRN2 and pCambia1301-35S-AsXRN2. Transgenic tomato lines were obtained via agrobacterium-mediated transformation, which would provide significant evidence for further study of XRN2 function in tomato