响应曲面分析法优化葡萄籽原花青素提取工艺的研究

摘要

The extraction technology of proanthocyanidins from grape seeds was studied using response Surface Method(RSM), and acetone concentration, solid-liquid ratio, temperature and time were selected as experimental the factors. Based on the analysis of significance and interaction among different factors, the optimal condition of extraction technology was obtained as followed: acetone concentration was 75％, solid-liquid ratio was 1:10, time was 44 rain and temperature was 51 C, and the predicted value and measured value of proanthocyanidins yield was 2.90％ and 2.88％, and the purity was 55.92％.%采用响应曲面分析法对葡萄籽原花青素的提取工艺进行优化.对提取率影响因素进行单因素分析后,以Box-Behnken设计(BBD)评价提取剂浓度、料液比、提取时间和提取温度4个因素显著性和交互作用的分析,得出葡萄籽原花青素的最佳提取条件为:丙酮浓度75%,料液比1:10(w/y),时间44min,温度51℃.在该条件下,葡萄籽原花青素提取率的预测值为2.90%,验证为2.88%,纯度为55.92%.