To get new clues and explore the unknown function and mechanism of HbRZF, the proteins that interacting with HbRZF were isolated and identified by the yeast two-hybrid system. HbRZF was amplified from Hevea brasiliensis by the PCR and then inserted into the pGBKT7 vector. After confirmed by sequence analysis, the plasmids were transformed into the cell of yeast strain Y2H Gold, and tested in terms of toxic and transcriptional activation by nutrition disfigurement assay. As a result, HbRZF was successfully amplified and cloned into the pGBKT7 vector. The yeast strain Y2H Gold cells harboring bait plasmids grew well on SD/-Trp plate, without toxicity and autonomous activation effects. Using HbRZF as the bait, the latex cDNA library was screened. The positive clones were sequenced and further analyzed. Sixteen positive clones that deduced to interact with HbRZF were identified. Given the significant functional correlation between HbRZF and its interacting proteins, it will help to elucidate the possible mechanisms of HbRZF in rubber tree.%为深入研究巴西橡胶树乳管细胞中C3HC4型环锌指蛋白HbRZF的生物学功能,构建了pGBKT7-HbRZF诱饵表达载体,利用酵母双杂交技术从巴西橡胶树胶乳cDNA文库中筛选与其相互作用的蛋白.通过阳性克隆的表型确定、PCR测序和生物信息学分析,初步获得了16个与诱饵蛋白相互作用的靶蛋白.获得乳管细胞中与HbRZF相互作用蛋白,为进一步研究HbRZF锌指蛋白在巴西橡胶树乳管细胞中的未知生物学功能奠定了重要基础.
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