巴西橡胶树脱水素基因HbDHN2的克隆及其氨基酸序列分析

摘要

脱水素(dehydrin， DHN)是存在于植物体内高度亲水的一类蛋白质，在植物响应和适应干旱、低温和盐渍等非生物胁迫过程中发挥重要作用。本研究在橡胶树已有转录组测序组装序列(TSA)数据的基础上，通过RT-PCR方法克隆了一个新巴西橡胶树(Hevea brasiliensis)脱水素基因 HbDHN2，并分析其氨基酸序列。结果表明，HbDHN2含210个氨基酸残基，与HbDHN1同为SK2型酸性脱水素，属于优先响应低温胁迫的脱水素类型(SKn)。%Dehydrins (DHNs) are a kind of highly hydrophilic plant proteins, which play a fundamental role in plant response and adaptation to drought, cold, salinity and other abiotic stress. A novel dehydrin gene, HbDHN2, was cloned from Hevea brasiliensis (rubber tree) by RT-PCR method and with the help of Transcriptome Shotgun Assembly (TSA) database of H. brasiliensis, and the amino acid sequence of HbDHN2 was analyzed. HbDHN2 contains 210 amino acid residues. Both HbDHN1 and HbDHN2 are SK2-type acidic dehydrins, and SKn-type dehydrins are preferentially induced by low temperature. In-depth study of the correlation between dehydrin and stress tolerance in rubber tree will be helpful for the breeding of new varieties of rubber tree.