巴西橡胶树乙醇酸氧化酶HbGOX1基因的鉴定与表达分析

摘要

Photorespiratory pathway is an important metabolic pathway in plant,which consumes the products of photo-synthesis and reduces crop final yield. Glycolate oxidase is the key regulatory enzyme of this pathway, while the study in rubber tree is absence. In this study, the cDNA sequence of HbGOX1 gene was identified and characterized, and the peptide sequence was analyzed bioinformatically. The expression pattern of HbGOX1 gene was further explored under cold stress. The results showed that this gene was negatively regulated by cold stress. This study provided some fundenmental information for the roles of photorespiration in rubber biosynthesis in Hevea brasiliensis.%光呼吸途径是植物中重要的反应途径，它通过消耗光合作用产物影响作物的产量。乙醇酸氧化酶是该途径的关键调控酶，在橡胶树中尚未见研究报道。本研究分离和鉴定橡胶树HbGOX1基因的全长cDNA序列，对其编码的蛋白质进行生物信息学分析，并通过qPCR技术进一步研究HbGOX1基因在低温胁迫下的表达情况。结果发现，该基因受低温胁迫负调控。此结果为进一步揭示橡胶树光呼吸途径在橡胶合成中的作用奠定了基础。