背景:研究表明急性心肌梗死后血管新生过程受众多基因调控.目的:观察急性心肌梗死后与血管新生途径相关的差异基因的表达.方法:建立急性大鼠心肌梗死模型,在基因芯片结果基础上选取5 个特定的表达差异基因:分泌磷酸蛋白1、趋化因子受体2、人血管生成素样蛋白4、CXC 趋化因子配体5 和白细胞介素1β.采用实时PCR 法验证5 个基因在心肌梗死大鼠模型正常组织及心肌梗死组织中的表达.结果与结论:分泌磷酸蛋白1、趋化因子受体2 和人血管生成素样蛋白4 在心肌梗死急性期表达上升显著(P < 0.05),呈时间依赖性.CXC 趋化因子配体5 和白细胞介素1β 基因表达水平呈低位波动,变化不显著.其中分泌磷酸蛋白1 与趋化因子受体2 与急性心肌梗死后炎性、细胞黏附、迁移和趋化相关,而人血管生成素样蛋白4 与血管新生和细胞分化相关.%BACKGROUND: Researches have shown that the process of angiogenesis after acute myocardial infarction is mediated by a number of genes.OBJECTIVE: To investigate the differential expression of angiogenesis related genes after acute myocardial infarction. METHODS: Rat model of acute myocardial infarction was constructed; five specific differential expression genes in gene chip were selected based on the results of gene chips: secreted phosphoprotein 1, chemokine receptor 2, angiopoietin-like 4, CXC chemokine ligand 5 and interleukin-1 β. Real-time PCR was conducted to detect the expression levels of these genes in normal and in infarction myocardial tissues of rat acute myocardial infarction model.RESULTS AND CONCLUSION: The gene expression of the secreted phosphoprotein 1, chemokine receptor 2 and angiopoietin-like 4 increased significantly in the early stage of acute myocardial infarction (P < 0.05); and it was time-dependent. While the expression levels of CXC chemokine ligand 5 and interleukin-1 β showed low fluctuation without significant changes. Among them, the secreted phosphoprotein 1 and chemokine receptor 2 are related to the inflammation, cell adhesion, migration and chemotaxis after acute myocardial infarction; while the angiopoietin-like 4 is related to angiogenesis and cell differentiation.
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