重组人白细胞介素1β对人牙髓细胞蛋白质组的影响

摘要

目的 创建牙髓损伤模型,比较牙髓细胞在不同条件下蛋白质表达的异同；探讨重组人白细胞介素1β(recombinant human IL-1β,rhIL-1β)对体外培养的人牙髓细胞(human dental pulpcells,HDPC)蛋白质的影响及牙髓损伤机制.方法 在HDPC的培养液中加入rhIL-1β 12 h,用双向凝胶电泳分离HDPC全蛋白,获得对照组(未加rhIL-1β)及诱导组(加入rhIL-1β诱导)HDPC蛋白质图谱,Image Master2D Elit 5.0软件分析确认差异表达蛋白.结果 诱导组牙髓细胞中有39个蛋白质点差异明显,其中15个蛋白质点高表达,新增13个蛋白质点,7个蛋白质点低表达,4个蛋白质点仅在对照组中表达.结论 rhIL-1β诱导HDPC后有多种蛋白质分子发生变化,双向凝胶电泳能够显示HDPC的全蛋白,而且能够发现差异表达蛋白质,这些差异表达蛋白质可能参与了牙髓细胞损伤的过程.%Objective To compare the similarities and differences of the dental pulp cells in protein expression under different conditions and to investigate the effect of recombinant human interleukin-1β ( rhIL -1 β) on the production of protein by human dental pulp cells (HDPC) in vitro.Methods HDPC were induced with rhIL-1 β for 12 hours and the dental pulp cell entire protein was separated by a 2-DE technique.The rhIL-1β induction and the normal dental pulp cell protein 2-DE atlas were established.Difference expression protein was confirmed by Image Master 2D Elite 5.0 software analysis.Results In induced dental pulp cells,39 protein spots were obviously different,of which 15 points were up-regulated,13 were new protein spots,7 points were down-regulated,4 points expressed only in the control group.Conclusions Two-dimensional gel electrophoresis can not only show the whole protein of human dental pulp cells are likely to be involved in the process of dental pulp cell damage.