Zyflamend对口腔黏膜癌变过程中前列腺素E2和白三烯B4水平影响的动物实验

摘要

目的 通过测定抗炎天然植物混合剂Zyflamend对地鼠口腔黏膜癌变过程中花生四烯酸代谢产物前列腺素( prostaglandin,PG) E2和白三烯B4水平的影响,探讨其可能的作用机制.方法 将27只金黄地鼠分为5组,阴性对照组3只,不涂药；其余24只用0.5％的二甲基苯并蒽( dimethylbenzanthracene,DMBA)涂于左侧颊囊3次/周,3周后将其按随机数字表随机分为4组(每组6只),阳性对照组不涂药,其余3组金黄地鼠分别于左侧颊囊分别涂低、中、高浓度Zyflamend(稀释比例分别为1∶3、1∶1和原液).每周涂药3次,第4周末处死动物取左侧颊囊速冻于液氮中行液相色谱-串联质谱法(liquid chromatographic tandem mass spectrometry,LC-MS/MS)分析.结果 阳性对照组PGE2为(0.377±0.290) μg/g,低、中、高浓度Zyflamend组的PGE2水平分别为(0.523±0.286)、(0.488±0.147)和(0.774±0.314) μg/g,低、中浓度组与阳性对照组相比差异无统计学意义(P＞0.05),高浓度组与阳性对照组相比差异有统计学意义(P＜0.05).与阳性对照组(0.135±0.046) μg/g相比,低、中、高浓度组白三烯B4水平均显著降低(P＜0.05),分别为(0.094±0.066)、(0.096±0.077)和(0.067±0.012) μg/g.结论 癌前病变的早期炎症阶段,Zyflamend能够抑制5-脂氧合酶途径中代谢产物白三烯B4的水平.%Objective To determine the level of prostaglandin E2 (PGE2 ) and leukotriene B4 (LTB4 ) after treatment with a unique anti-inflammatory herbal preparation (Zyflamend) in 7,12-dimethylbenz(a) anthracene (DMBA)-induced oral precancerous lesions in golden Syrian hamsters.Methods The hamsters were divided into five groups.The Syrian hamsters were applied with 0.5％ DMBA solution topically to the left cheek pouch three times per week for three consecutive weeks.The negative control group was not treated.After the last treatment by DMBA,the 0.5％ DMBA treated hamsters were divided into four groups at random.The positive group was not treated,the other groups received low,middle and high doses of Zyflamend respectively three times within the following week.The animals were sacrificed 4 weeks after the start of the experiment.They were injected with 5-bromo-2'-deoxyuridine (BrdU) 50 mg/kg before they were killed.A piece of tissue was snap-frozen in liquid nitrogen for liquid chromatographic tandem mass spectrometry analysis.Results The level of PGE2 was increased from (0.377 ±0.290) μg/g to (0.523 ±0.286),(0.488 ±0.147) and (0.774 ±0.314) μg/g.There was no significant difference between positive group and groups with low,middle doses of Zyflamend ( P ＞ 0.05 ).The level of PGE2 was significantly increased in the treatment group of high dose of Zyflamend.Low,middle and high doses of Zyflamend significantly decreased the LTB4 level from (0.135 ±0.046)to (0.094 ±0.066),( 0.096 ± 0.077 ) and ( 0.067 ± 0.012 ) μg/g respectively ( P ＜ 0.05 ).Conclusions Zyflamend have inhibitory effect on oral carcinogenesis.