目的 评价聚酰胺-胺对纯钛表面白色念珠菌和牙龈卟啉单胞菌黏附的影响.方法 将64个纯钛试件采用抽签法随机分为8组.A、A1为对照组,B、B1聚酰胺－胺处理,C、C1唾液处理,D、D1聚酰胺-胺+唾液处理,A、B、C、D4组试件加入2 ml白色念珠菌菌液；A1、B1、C1、D1组试件加入牙龈卟啉单胞菌液.试件于白色念珠菌和牙龈卟啉单胞菌液中培养,染色,荧光观察.结果A、B、C、D4组白色念珠菌黏附量值为227.1±17.6、175.4±17.0、340.7±26.0和283.5±27.8,析因设计方差分析结果显示聚酰胺－胺处理组的菌黏附量减少(F=46.405,P＜0.001)；A1、B1、C1、D1组黏附的牙龈卟啉单胞菌活菌率为(59.42±2.76)％、(40.97±1.93)％、(63.03±1.99)％和(43.92±2.27)％,聚酰胺-胺处理组的活菌率低于未处理组(F =552.037,P＜0.001).结论聚酰胺－胺对纯钛表面黏附的白色念珠菌和牙龈卟啉单胞菌均有抑制作用.%Objective To evaluate the influence of polyamidoamine dendrimer on the adhesion of Candida albicans ( Ca ) and Porphyromonas gingialis ( Pg ) to titanium specimens.Methods Sixty-four titanium specimens ( 10 mm × 10 mm × 1 mm) were prepared and divided into eight groups of A,B,C,D,A1,B1,C1 and D1.Group A and A1 were the control groups.B and B1 were treated with polyamidoamine dendrimer,C and C1 were coated with saliva,D and D1 were treated with polyamidoamine dendrimer and saliva.The specimens were incubated in Ca and Pg cell suspension,and then fluorescence stained,photographed by fluorescence microscopy.Results Number of adherent Ca on A,B,C and D is 227.1 ±17.6,175.4 ± 17.0,340.7 ± 26.0 and 283.5 ± 27.8 respectively.The main effect of pellicle was statistically significant ( F =191.973,P ＜ 0.001 ).Adherent Ca on the surfaces of specimens coated with saliva were more than those on the specimens coated without saliva.The main effect of polyamidoamine dendrimer was statistically significant ( F =46.405,P ＜ 0.001 ).The number of adherent Ca on specimens treated with polyamidoamine dendrimer was less than that in control group ( P ＜ 0.05 ).The percentages of vital adherent C.a were (57.07 ± 1.83 ) ％,(44.18 ± 2.72) ％,(59.04 ± 2.44) ％ and (45.89 ± 3.19 ) ％respectively.The main effect of pellicle was not statistically significant ( F =4.043,P ＞ 0.05 ).The main effect of polyamidoamine dendrimer was statistically significant ( F =202.066,P ＜ 0.001 ).The percentage of vital adherent Ca on specimens treated with polyamidoamine dendrimer was lower than that in control group.The number of adherent Pg on surfaces of Group A1,B1,C1 and D1 was 536.78 ±30.27,336.15 ± 26.13,1219.20 ± 172.04,and 859.85 ± 47.04 respectively.There was statistically significant interaction between the pellicle and polyamidoamine dendrime (F =4.350,P ＜ 0.05 ).The pellicle enhanced the effect of polyamidoamine dendrime inhibiting pg adhesion,while the polyamidoamine dendrime weakened the effect of pellicle.The percentages of vital adherent bacterias of group A1,B1,C1 and D1 were (59.40 ± 2.77 ) ％,(40.97 ± 2.50) ％,( 63.04 ± 1.99) ％ and (43.93 ± 3.11 ) ％ respectively.The values of groups coated with saliva were higher than the non-coated ( F =16.903,P ＜ 0.001 ).The values of groups treated with polyamidoamine dendrimer were lower than non-treated ( F =552.037,P ＜ 0.001 ).Conclusions Polyamidoamine dendrimer can inhibit the adhension of Ca and Pg on titanium materials.
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