补锌对耐力训练、力竭运动大鼠腓肠肌超氧化物岐化酶、谷胱甘肽过氧化物酶、总抗氧化能力以及Bax、Bcl-2mRNA的影响

摘要

Objective The experiment aims at studying the effects of Zinc supplementation (ZS) on the superoxide dismutase (SOD) , glutathione peroxidase (GPX) , total antioxidant capacity (T-AOC) , BAX and Bcl-2 mRNA after endurance training and acute exhaustive exercise in SD rats' gastrocnemius. Methods Thirty two male SD rats were randomly divided into control group (C) , endurance training group (T) , endurance training with ZS group (E) and acute exhaustive exercise with ZS group (O) . Drinking water containing ZnSO4·7H2O ( [Zn] =227mg/L) was used to supply groups E and O. Rats in groups T and E swam 6 days per week for 6 weeks, and their gastrocnemius was sampled 24 hours after the last training. Two rats every 15 minutes underwent acute exhaustive exer-cise (swam without loading until exhaustion) and sampling was performed immediately after exhaustion. SOD. GPX, T-AOC, and Bax, Bcl-2 mRNA level of gastrocnemius were measured. Results The activity of SOD in group O is obviously higher than that in group C (P < 0.05) , and the activity of GPX in group O is higher than those in groups C and T ( P < 0.05 ) .The activity of GPX and T-AOC in group O is obviously higher than that in group C (P < 0.05 ) . The mRNA level of Bax in group E is obviously lower than those in groups C and T (P < 0.05 ) , and the mRNA level of Bcl-2 is obviously lower than those in groups C and T. The mRNA levels of Bax and Bcl-2 in group O are both higher than that in group C. Conclusions Endurance training increases the activity of GPX and the level of Bcl-2 mRNA, and decreases the level of Bax mRNA in rats' gastrocnemius. Zinc supplementation appears to be effective. Acute exhaustive exercise with zinc supplementation increases the activity of GPX, T-AOC and mRNA level of Bcl-2 in rats' gastrocnemius and less affects the mRNA level of Bax.%目的:探讨补锌对耐力训练、力竭运动大鼠腓肠肌超氧化物岐化酶( SOD)、谷胱甘肽过氧化物酶(GSH-Px,简称GPX)、总抗氧化能力(T-AOC)、细胞凋亡诱导和阻遏分子(Bax,Bcl-2) mRNA的影响.方法:将32只雄性SD大鼠随机分成对照组、耐力训练组、耐力训练补锌组和一次性力竭运动补锌组4组,每组8只.补锌方法为在饮用水中溶入ZnSO4·7H2O,[Zn]=227 mg/L.耐力训练组和耐力训练补锌组进行6周游泳耐力训练,每周6天.6周训练结束24小时后取材.一次性力竭运动组取材当日在同一泳池每隔15 min被放入2只大鼠,进行无负重游泳至力竭后取材.测定大鼠腓肠肌SOD、GPX、T-AOC活性,Bax、Bcl-2 mRNA表达.结果:耐力训练补锌组大鼠腓肠肌SOD活性显著高于对照组(P＜0.05),GPX活性显著高于对照组和耐力训练组(P＜0.05).力竭运动补锌组大鼠腓肠肌GPX和T-AOC活性显著高于对照组(P＜0.05).耐力训练补锌组大鼠腓肠肌Bax mRNA表达显著低于对照组和耐力训练组(P＜0.05),Bcl-2 mRNA表达显著高于对照组和耐力训练组(P＜0.05).力竭运动补锌组大鼠腓肠肌Bax,Bcl-2 mRNA表达均显著高于对照组(P＜0.05).结论:耐力训练提高大鼠腓肠肌GPX活性,增加Bcl-2 mRNA表达,降低大鼠腓肠肌Bax mRNA表达,补锌效果更明显.一次性力竭运动补锌提高大鼠腓肠肌GPX活性和T-AOC活性,增加腓肠肌Bcl-2 mRNA表达,但对Bax mRNA表达影响不明显.