有氧运动通过胃促生长素调节肥胖及肥胖抵抗大鼠摄食量的研究

摘要

目的:探讨有氧运动对肥胖及肥胖抵抗大鼠摄食量的影响及机制.方法:4周龄雄性Sprague-Dawley大鼠,随机分为普饲对照组(C组)和高脂饲料组,饲养10周后,筛选出高脂饮食诱导的肥胖组(OB组)大鼠和肥胖抵抗组(OR组)大鼠.再分别将OB组和OR组随机分为运动组(OB-Sw组,OR-Sw组)大鼠和非运动组(OB-Sed组,OR-Sed组)大鼠,运动组大鼠进行8周60％的个体力竭强度的有氧游泳运动,45min/d,5次/周,每周监测大鼠体重和摄食量.8周干预结束后,取血清和胃组织,血清检测TG、TC、LDL-c、HDL-c,胃组织检测促生长素(Ghrelin)表达情况.结果:10周后,高脂饲料组大鼠体重与普饲对照组相比,显著性增高(P＜ 0.05);OB-Sed组大鼠的血清低密度脂蛋白(LDL)与C组的相比,显著性增高(P＜0.05);通过8周的有氧运动,OB-Sed组大鼠的相对摄食量多于OB-Sw组,OR-Sed组大鼠的相对摄食量多于OR-Sw组;OB-Sed组、OR-Sed组大鼠胃组织Ghrelin表达程度高于OB-Sw组、OR-Sw组和C组.结论:运动可通过降低大鼠胃组织Ghrelin的表达来降低摄食量.%Objective:To explore the effect of aerobic exercise on the food intake of obesity rats and obesity resistant rats and some mechanisms Method:The four years old Sprague-Dawley rats were randomly divided into standard chow group (C) and high-fat diet group.After 10 weeks feeding,the obese group (OB group) and obesity resistant group (OR group) induced by high-fat diet were screened out.Then all the rats in OB group and OR group were respectively and randomly divided into exercise rats (OB-Sw group,OR-Sw group) and non-exercise rats (OB-Sed group,OR-Sed group).Aerobic swimming exercise for the exercise rats was defined as 60％ of individual exhaustion exercise intensity,45 minutes per day,5 times per week.Meanwhile,the body weight and food intake of the exercise and non-exercise rats were monitored every week.After 8 weeks intervention,the serum and gastric tissue of rats were obtained,and then the TG,TC,LDL-c,HDL-c in serum and the expression of ghrelin in gastric tissue were detected.Result:Ten weeks later,the body weight of the rats with high-fat diet showed a significant difference compared with the rats with standard chow group (P ＜ 0.05).The content of LDL in OB-Sed group was significantly higher than that in C group (P＜0.05).After 8 weeks aerobic exercise,for the food intake,OB-Sed group was higher than OB-Sw group and OR-Sed group was higher than OB-Sw group.The expression of ghrelin in gastric tissue in OB-Sed group and OR-Sed group was higher than that in OB-Sw group,OB-Sw group and C group.Conclusion:Exercise might reduce the food intake of obesity rats and obesity resistant rats by reducing the expression of ghrelin in stomach.