精神分裂症患者儿茶酚-O-甲基转移酶基因Val108/158Met多态性与P50感觉门控的关系

摘要

目的 探讨精神分裂症患者儿茶酚-0-甲基转移酶(catechol-O-methyl transferase,COMT)第158位密码子从缬氨酸到蛋氨酸的多态性(Val108/158 Met)与P50感觉门控的关系.方法 共纳入精神分裂症患者139例(患者组),健康对照165名(对照组),对所有研究对象进行事件相关电位(event-related potential,ERP)检测,以S1、S2、S1-S2、S2/S1为指标评价P50感觉门控;应用聚合酶链反应-限制性片段长度多态性(polymerase chain reaction-restriction fragment length polymorphism,PCR-RFLP)方法,分析患者组与对照组COMT Val 108/158Met基因型与等位基因分布频率,比较2组不同基因型间P50各指标的差异.结果 (1)患者组S1(2.84±1.53)、S1-S2(1.03±1.61)较对照组(3.63±1.94、2.10±1.77)低(t=-3.96、-5.45,均P＜0.05),S2/S1 (0.83±0.96)较对照组(0.48±0.52)高(t=3.95,P＜0.05),2组S2差异无统计学意义(1.81±1.15与1.53±1.39,t=1.876,P＞0.05);(2)患者组Val/Val、Val/Met、Met/Met基因型间S1 (2.87±0.22、2.85±0.19、2.72±0.38)、S2(1.88±0.16、1.82±0.14、1.53±0.28)、SI-S2(0.98±0.23、1.03±0.20、1.19±0.40)、S2/S1 (0.83±0.13、0.89±0.12、0.63±0.24)的差异均无统计学意义(F=0.06、0.59、0.11、0.51,均P＞0.05),男性患者Val/Val、Val/Met、Met/Met基因型间S1(2.59±0.24、2.77±0.19、2.62±0.39)、S2(1.78±0.18、1.77±0.14、1.21±0.30)、S1-S2(0.81±0.27、1.00±0.21、1.41±0.43)、S2/S1[0.87±0.19、0.94±0.15、0.49±0.32)的差异均无统计学意义(F=0.20、1.61、0.71、0.86,均P＞0.05);(3)2组间COMT基因型与等位基因分布差异有统计学意义(X2=7.74、5.87,均P＜0.05),未发现诊断和COMT基因型的交互作用与S1、S2、S1-S2和S2/S1相关联(F=0.20、0.10、0.40、0.33,均P＞0.05).结论 COMT基因Val108/158 Met多态性可能与精神分裂症P50感觉门控异常无关.%Objecttive To investigate the association between catechol O-methyl transferase (COMT) Val108/158Met polymorphism and P50 sensory gating in patients with schizophrenia.Methods In this study,event-related potentials (ERP) was determined in 139 stable patients with schizophrenia and 165 healthy controls.P50 sensory gating was evaluated by S1,S2,S1-S2,and S2/S1.COMT Va1108/158 Met polymorphism was genotyped by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).The differences of P50 gating among COMT genotypes were compared.Results Compared with control group,schizophrenic patients exhibited smaller S1,S1-S2,and a higher S2/S1 ratio (2.84±1.53,1.03±1.61,0.83±0.96 vs.3.63± 1.94,2.10± 1.77,0.48±0.52,t=-3.96,-5.45,3.95,all P＜0.05).There was no difference in S2 between schizophrenic patients (1.81 ± 1.15) and control group (1.53± 1.39,t=1.876,P＞0.05).The significant differences in COMT allelic and genotypic distributions were observed between schizophrenic patients and control group (X2=7.74,5.87,all P＜0.05).There were no differences in S1 (2.87± 0.22,2.85±0.19,2.72±0.38),S2 (1.88±0.16,1.82±0.14,1.53±0.28),S1-S2 (0.98±0.23,1.03±0.20,1.19± 0.40),and S2/S1 (0.83±0.13,0.89±0.12,0.63±0.24) among COMT genotypes for schizophrenic patients (F=0.06,0.59,0.11,0.51,all P＞0.05).No differences were also observed in S1 (2.59±0.24,2.77±0.19,2.62± 0.39),S2 (1.78±0.18,1.77±0.14,1.21±0.30),S1-S2 (0.81±0.27,1.00±0.21,1.41±0.43),and S2/S1 (0.87±0.19,0.94±0.15,0.49±0.32) among COMT genotypes for men patients (F--0.20,1.61,0.71,0.86,all P＞0.05).The significant differences in COMT allele and genotype distributions were observed between schizophrenic patients and control group (X2=7.74,5.87,all P＜0.05).The interaction between diagnosis and COMT genotype did not seem to associate with S1,S2,S1-S2 and S2/S1 (F=0.20,0.10,0.40,0.33,all P＞0.05).Conclusion COMT Va1108/158 Met polymorphism may not be associated with P50 sensory gating abnormalities of schizophrenia among Han Chinese.