为同源表达鼠伤寒沙门氏菌(S.typhimurium)的lsrB基因,本研究通过PCR扩增得到S.typhimurium SL1344株的lsrB基因,其全长1020 bp,编码340个氨基酸.通过序列分析,该基因与S.typhimurium LT2的neA基因同源性为100％,与克雷伯氏菌(Klebsiella) AI-2结合蛋白基因lsrB同源性为77％.以pBAD24为载体构建得到在其C端含有Strep-tag的LsrB融合蛋白表达重组质粒pBAD24-lsrB.将重组表达质粒转化同源菌株SL1344中,经阿拉伯糖诱导表达后,采用SDS-PAGE与western blot检测结果表明:表达的融合蛋白约为37 ku;并初步证明该蛋白为非分泌型蛋白.本研究为进一步研究LsrB的功能及其表达调节奠定了基础.%The lsrB gene in Salmonella typhimurium SL1344 was amplified using PCR amplification and the PCR product size was 1,020 bp, encoding 340 amino acids. The similarity of lsrB gene to yneA gene in S. Typhimurium LT2 and to lsrB gene in Klebsiella was 100% and 77%, respectively. The recombinant plasmid of pBAD24-lsrB with Strep-tag was constructed and transformed into SL1344. The 37 ku recombinant LsrB was expressed in SL1344 induced with arabinose and was detected with SDS-PAGE and western blot, which demonstrated that LsrB in SL1344 might not be a secreted protein. This study could lay a foundation for further researching the function and regulation of lsrB in Salmonella.
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