抗Ⅰ型鸭肝炎病毒单克隆抗体的制备及其生物学特性鉴定

摘要

To prepare a monoclonal antibodiy (MAb) to duck hepatitis virus Ⅰ (DHV-1), 4 weeks to 6 weeks old female BALB/c mice were immunized by purified DHV-1.Four hybridomas secreting MAbs were selected by indirect-ELISA screening,designated AF8, BF5, BG6 and DB6.Dot-ELISA, IFA test analysis showed all the MAbs reacted with DHV-1 but no cross reaction to New-DHV.In addition, BF8 and BG6 showed virus neutralization activity.The preparation of the MAbs for DHV-1 established a good foundation for further studying DHV-1.%为制备抗Ⅰ型鸭肝炎病毒(DHV-1)的单克隆抗体(MAb),本研究采用纯化的DHV-1免疫4周龄～6周龄BALB/c雌鼠,按常规方法制备MAb,通过间接ELISA方法进行筛选获得4株能稳定传代并分泌抗DHV-1的MAb的杂交瘤细胞株,分别命名为AF8、BF5、BG6和DB6.Dot-ELISA、间接免疫荧光试验和中和试验等结果表明4株MAbs效价为1:1600～1:3200,与新型DHV无交叉反应.其中,BF5和BG6具有中和活性.抗DHV-1的MAb的制备为DHV-1的生物学特性研究及其快速诊断方法的建立奠定了基础.