高效液相色谱法测定小鼠胰腺组织中头孢匹胺浓度

摘要

Objective To establish a reversed-phase HPLC(RP-HPLC) technique for the quantification of the cefpiramide (CPM) concentration in pancreatic tissue. Methods The following RP-HPLC conditions were set up DISCOVERY ODS C18 column(5μm, 250 mm×4.6 mm) as stationary phase; a mixture of methanol, triethanolamine and water(27-0.2-72.8) at a flow rate of 1.0 mL·min-1 as mobile phase; UV detection at 254 nm. Results CPM concentration of pancreatic tissue showed a linear plot in the range of 0.1～500.0μg·Ml-1(r=0.999 8). The minimal quantitative concentration was 0.1 μg·Ml-1. The mean recovery at the quantitative concentrations of CPM was (97.18+0.74)%. The coefficients of variation for within-day performance were found to be less than 5%, and between-day performance less than 10%. Conclusion The RP-HPLC is simple and sensitive to quantify cefpiramide concentration of pancreatic tissue, and may be used to monitor the PK parameters in tissues.%目的 建立高效液相色谱法(HPLC)测定小鼠胰腺组织中头孢匹胺浓度的方法.方法 以DISCOVERY ODS C18柱(5 m,250×4.6 mm)为色谱柱,甲醇三乙胺醋酸水(27 0.2 72.8,v/v)为流动相,流速1.0 mL·min-1,柱温30℃,紫外检测波长254nm.结果 小鼠胰腺组织中的内源性物质不干扰头孢匹胺的测定,头孢匹胺在0.1~500.0μg·mL-1浓度范围内峰面积与浓度线性关系良好(r=0.999 8),最低检测限为0.1μg·mL-1.平均回收率为(97.18±0.74)%,高、中、低3种浓度的日内差(RSD)<5%,日间差(RSD)<10%.结论 本方法简便、快速、准确,重现性好,可应用于头孢匹胺组织内药动学研究.