HPLC 法同时测定七味清咽气雾剂中苦参碱、射干苷和次野鸢尾黄素的含量

摘要

Objective To develop a HPLC method for determination of martine, belamcandin and irisflorentin in Qiweiqingyan aerosol. Method The optimum HPLC condition was as follows: Agilent Zorbas Stablebond C18 column (4.6 mm×250 mm,5 μm) with gradient elution of acetonituile (A)- methanol(B)-0.1% phosphoric acid (C), detection wavelength:251 nm, column temperature: 30℃, flow rate:1mL·min-1. Results There were good linearity between peak areas and injection quantity of martine, belamcandin and irisflorentin within the range of 0.25~125 μg·mL -1, 0.10~101μg·mL -1, 0.12~234μg·mL -1.The average recoveries were between 98.5 % ~100.6% . Conclusion The method could detect martine, belamcandin and irisflorentin in Qiweiqingyan aerosol. The HPLC measurement was reasonable, stable and accurate.%目的：建立 HPLC 法测定七味清咽气雾剂中苦参碱、射干苷和次野鸢尾黄素的含量。方法色谱柱为 Agilent Zorbas Stablebond C18柱（4.6 mm×250 mm，5μm）；流动相组成为乙腈（A）-甲醇（B）-0.1％磷酸水溶液（C）,梯度洗脱,检测波长为251nm，柱温为30℃，流速为1mL·min-1。结果苦参碱、射干苷和次野鸢尾黄素分别在0.25～125μg·mL-1，0.10～101μg·mL-1，0.12～234μg·mL-1范围呈良好的线性关系，与峰面积积分值的线性关系良好，平均回收率为98.5%～100.6%之间。结论本方法可用于测定七味清咽气雾剂中苦参碱、射干苷和次野鸢尾黄素的含量，结果准确，重复性好。