Betaine have protective effects on liver,cardiovascular system,and cancer,but there is no report about I/R injury in heart. So the purpose of this experiment is to study the effect of betaine on I/R injury by Langendorff model and to discuss the mechanisms. SD rat were randomly divided into 4groups: control group(perfused with KH buffer),10^(-4)mol·L^(-1),5×10^(-4)mol·L^(-1),and 10^(-3)mol·L^(-1)(perfused with betaine in different concentration respectively for 10 min before ischemia followed by KH buffer perfused). During the experiment we detected LVEDP,LVDP,dp/dt,and store the tissue to do western blot and TTC staining. The result showed that in control group the recovery rate is 19.6%,after pretreatment of betaine the recovery rate all increased significantly and in a dose-dependent manner. The LVEDP is markedly decreased in betaine treatment groups compare to control group. Coronary flow only in highest betaine treatment group(10^(-3)mol·L^-) is markedly increased compare to control group,other two groups have no change. Western blotting showed that SOD level is significantly increased in betaine treatment group compare to control group and in a dose dependent manner. TTC staining suggested that infarction size is reduced in betaine treatment group compare to control group. Above all,the results suggest that betaine have protect effect on I/R injury in heart and this effect may relate to ROS signaling pathway.
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