1-(2,6-二甲基苯氧基)-2-（3，4-二甲氧基苯乙氨基）丙烷盐酸盐对小鼠脑缺血再灌损伤的保护作用

摘要

采用断颅，iv饱和MgCl2溶液及结扎双侧颈总动脉和迷走神经等法造成小鼠脑缺血模型，观察1-(2,6-二甲基苯氧基)-2-（3，4-二甲氧基苯乙氨基）丙烷盐酸盐（DDPH）对小鼠脑缺血后存活时间的影响；采用小鼠脑缺血（20 min）再灌注(10 min)模型，观察DDPH对脑组织超氧化物歧化酶（SOD）活性, 丙二醛（MDA）含量及组织病理损伤的影响。结果显示，DDPH 3，6，12,24 mg*kg-1缺血前30 min ip给药使小鼠存活时间明显延长；使小鼠脑缺血再灌注后脑组织内SOD活性增高，MDA含量下降，并明显改善神经细胞的病理性损伤. 结果提示DDPH对小鼠脑缺血再灌注所致损伤具有一定的保护作用.%By using decapitating, intravenous injection of saturated MgCl2 and legation of bilateral carotid arteries with vagi, the effects of 1-(2,6-dimethylphenoxy)-2-(3,4-dimethoxyphenylethyl- amino) propane hydrochloride(DDPH) on survival time in mice were studied. With the model of cerebral ischemia for 20 min and reperfusion for 10 min, effects of DDPH on the superoxide dismutases(SOD) activity and malondialdehyde (MDA) content in brain tissue and pathological changes were studied. The results indicated that DDPH at dosages of 3,6,12,24 mg*kg-1 ip 30 min before ischemia prolonged the survival time significantly. Meanwhile, DDPH was found to increase the activity of SOD and reduce the content of MDA, as well as mitigate pathological damage of neuron after cerebral ischemia and reperfusion in mice. The results suggest that DDPH has protective effects on brain ischemia.