蟾酥对豚鼠心脏电生理的影响

摘要

目的 探讨蟾酥对豚鼠心脏的急性毒性.方法 豚鼠一次性ig给予蟾酥125和250 mg·kg-1,记录心电图并监测左心室内压力上升最大速率(dp/dtmax),最大收缩压(Pmax),心率血压乘积(RPP)和心室最小舒张压(Pmin).结果 蟾酥使豚鼠心电图发生显著改变;与正常对照组P-R间期(27.8±5.1)ms相比,蟾酥125和250 mg·kg-1组显著延长,分别为44.5±7.2和(57.1±8.9)ms(P<0.01);蟾酥也引起心电图QRS时程增宽和心率加快;dp/dtmax,Pmax和RPP显著下降以及Pmin显著升高(P<0.05).与阳性对照地高辛300 mg·kg-1相比,蟾酥组P-R间期,QRS时程,dp/dtmax,Pmax,RPP和Pmin无显著性差异,但心率显著增快(P<0.01).结论 蟾酥导致豚鼠心律失常和心功能下降.%OBJECTIVE To study the effect of Chansu (toad venom, Venenum Bufonis) on the cardiac electrical activity and function. METHODS After guinea-pigs were ig given a single dose of Chansu 125 and 250 mg· kg -1 , electrocardiography and cardiac function were monitored.The cardiac function was evaluated by measuring the maximum rate of rise of left ventricular pressure ( dp/dtmax ) , the maximum systolic pressure ( Pmax ) , rate-pressure product (RPP) and the minimum diastolic pressure (Pmin). RESULTS Compared with normal control group, the P-R interval in Chansu 150 and 250 mg·kg-1 groups significantly increased from (27.8 ±5.1 ) ms to 44.5 ±7.2 and (57.1 ± 8.9 ) ms ( P ＜ 0.01 ). Chansu also induced wide QRS complex and rapid heart rate, markedly increased Pmin ( P ＜ 0.05 ), but decreased Pmax, RPP and dp/dtmax (P ＜ 0.05 ). No significant difference in P-R interval, QRS complex, dp/dtmax, Pmax RPP and Pmin was observed between Chansu and digoxin 300 mg·kg-1, but Chansu induced faster heart rate than digoxin. CONCLUSION Chansu poisoning can induce arrhythmias and cardiac dysfunction.