OBJECTlVE To elucidate the sensitization mechanism of baicalin in guinea pigs. METHODS Baicalin antigens were prepared by activated ester method. A guinea pig model of allergic shock induced by the coupling was established. Passive cutaneous anaphylaxis ( PCA) was used to ana-lyze the regularity of the specific antibody. The lgG antibodies of baicalin in serum among 19 sensitized guinea pigs were determined by ELlSA. RESULTS UV scanning spectrum couplings occurred between baicalin and protein ( bovine serum albumin and human serum albumin) . The conjugation ratio was 9∶1 and 5∶1, respectively. The guinea pig model of allergic shock was successfully established and suggested that the antigen induced a typical immediate hypersensitivity response. The antibody could be determined on the 19th day and reached the maximum on the 31st day and then degraded gradually. Both specific lgE and lgG existed in the sensitized serum, but there was no correlation between lgG and lgE. The pos-itive rate of specific lgG antibody to baicalin was 84%. The degranulation rate of peritoneal mast cells was (72.6±11.4)%and higher than that incubated with plasma from control (26.8±6.9)%(P<0.05). lsolated ileum from sensitized guinea pigs contracted conspicuously after being challenged by baicalin-human serum albumin. CONCLUSlON Baicalin can induce allergic reaction in guinea pigs and the reaction is correlated with lgE and lgG production.%目的：探讨黄芩苷的致敏作用机制。方法采用活性酯法制备黄芩苷全抗原，建立黄芩苷致豚鼠过敏性休克模型；采用被动皮肤过敏反应( PCA)实验分析豚鼠特异性抗体产生的规律性以及lgE和lgG抗体效价，采用ELlSA测定豚鼠血清中黄芩苷特异性lgG抗体。结果成功合成黄芩苷全抗原，黄芩苷与牛血清蛋白( BSA)和人血清白蛋白( HSA)的偶联效率分别为9∶1和5∶1。成功建立黄芩苷致敏的豚鼠休克模型，并显示典型的速发型过敏反应特征。致敏豚鼠第一次致敏后第19天开始用PCA实验检测出抗黄芩苷抗体，第31天达高峰，之后逐渐降低。致敏豚鼠体内同时存在lgE和lgG型特异性抗体，且两抗体之间无明显相关性。致敏豚鼠血清中黄芩苷特异性lgG抗体的阳性率为84％，致敏组肥大细胞脱颗粒率为(72.6±11.4)％，显著高于阴性对照组(26.8±6.9)％。致敏豚鼠离体回肠经黄芩苷-HSA激发后收缩明显。结论黄芩苷能够导致豚鼠出现过敏反应，并与特异性lgE和lgG抗体有关。
展开▼
