OBJECTIVE To investigate the inhibitory effects of extracts of Flos Chrysanthemi indici (FCI) on hyperglycemia,hyperipidemia and aldose reductase (AR) in diabetic KKAy mice. METHODS Twenty-eight male KKAy mice of 8 weeks old were randomly divided into four groups according to the initial fasting glucose:KKAy model group,extract of FCI 20,100 mg · kg-1 groups and glimepiride 0.4 mg · kg-1 group. C57BL/6J mice were taken as the normal control group. These mice were given ig once daily for 7 weeks. The body mass and food intake were recorded weekly. The fasting glucose and OGTT were measured in the last week of the experiment. Mice were sacrificed 1 d after the last admin⁃istration. The indexes of blood biochemistry were determined. Serum insulin,leptin and AR levels were analyzed by immunoassay using ELISA kits. The weights and pathological changes the in the liver,kidney, spleen,including epididymal and perirenal adipose,were measured. The expression of AR mRNA in the kidney was detected by real time-PCR. RESULTS Compared with model control group,the food intake,fasting glucose and AUC in OGTT significantly decreased(P<0.01),the serum glutamic-pyruvic transaminase,glucose,triglycerides,total cholesterol,low-density lipoprotein cholesterol,insulin, leptin and AR levels were markedly reduced(P<0.05),and the organ indexes of the liver and kidney sig⁃nificantly decreased(P<0.05)in extracts of FCI 100 mg · kg-1 group. The histopathological changes in model group included tubular epithelial cell degeneration,hepatic steatosis,islet β-cell degeneration, spleen white pulp atrophy and hypertrophy of the adipocyte. Symptoms listed above were attenuated by extracts of FCI and glimepiride treatment. The inhibitory effects of FCI also inhibited the expression of AR mRNA in the kidney. CONCLUSION The extract of FCI has hypoglycemic and hypolipidemic effect and can inhibit AR in KKAy mice,which may protect the kidney,liver,adipose,pancrea and spleen from the damage of diabetes.%目的：观察野菊花提取物对糖尿病KKAy小鼠血糖和血脂水平的影响，及对醛糖还原酶（AR）的抑制作用。方法8周龄雄性KKAy小鼠，根据初始空腹血糖随机分为模型组、野菊花提取物20和100 mg·kg-1组及格列美脲0.4 mg·kg-1组，以雄性C57BL/6J小鼠作为正常对照组，每天ig给药1次，连续7周。每周测量小鼠体质量和摄食量，实验结束前测定空腹血糖并进行口服葡萄糖耐量实验（OGTT）。末次给药后禁食过夜，处死小鼠，取血清进行生化检测，ELISA测定血清中胰岛素、瘦素和AR水平，解剖取肝、肾、脾、睾周脂肪和肾周脂肪，测定脏器系数并进行常规病理组织学检查。实时PCR测定肾组织匀浆中AR mRNA表达。结果与模型组比较，野菊花提取物100 mg· kg-1组小鼠摄食量明显减少（P<0.01），空腹血糖明显降低（P<0.05）；OGTT结果表明野菊花提取物可显著抑制葡萄糖吸收（P<0.01），血清谷丙转氨酶、葡萄糖、甘油三酯、胆固醇、低密度脂蛋白胆固醇、胰岛素、瘦素和AR水平明显降低（P<0.05，P<0.01）。组织病理学观察显示，模型组小鼠肝、肾、脂肪、胰腺和脾组织出现不同程度病变，野菊花提取物可抑制糖尿病引起的组织病理变化；同时可降低肾组织AR mRNA表达。结论野菊花提取物对KKAy小鼠有降血糖和血脂的作用，并能保护因糖尿病引起的肾、肝、脂肪、胰腺和脾损伤，其对肾的保护作用与降低肾组织AR mRNA表达从而抑制AR作用有关。
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