氨磷汀对人巨核细胞白血病Dami细胞增殖及分化的影响

摘要

OBJECTIVE To investigate the effect of amifostine(Amf)on the differentiation of human megakaryocyte cell line-Dami. METHODS Dami cells were treated with Amf 0.01-5.0 mmol · L-1 for 12 d. Dami cells were counted every day for the growth curve:only cells with a diameter>20μm. The platelet demarcation membrane system was observed by transmission electron microscopy. The expression of CD33,CD34,CD41a and DNA ploidy was detected by flow cytometry. RESULTS Amf 0.1-1.0 mmol · L-1 promoted the differentiation of Dami cells ,but inhibited their proliferation at a concentration>1.0 mmol · L-1. When these cells were treated with Amf 1.0 mmol · L-1 for 12 d,the platelet demarcation membrane system was observed,the percentage of cells with a diameter >20 μm was increased by 24.6%(P1.0 mmol·L-1).%目的：探讨氨磷汀（依硫磷酸，Amf）对人巨核细胞白血病Dami细胞分化的影响。方法 Amf 0.01~5.0 mmol·L-1分别处理Dami细胞12 d，每天光镜下观察细胞形态，计数Dami细胞数量，绘制增殖曲线；第12天，计数直径>20μm细胞数量，透射电镜观察Dami细胞血小板分界膜系统的形成，流式细胞仪检测细胞DNA倍体化及细胞表面抗原CD33，CD34和CD41a的变化。结果 Amf 0.1~1.0 mmol·L-1促进Dami细胞分化；Amf>1.0 mmol·L-1时抑制Dami细胞增殖，Amf 1.0 mmol·L-1作用12 d后光镜下观察到细胞体积增大，直径>20μm细胞比例增加24.6%（P1.0 mmol·L-1时抑制Dami细胞增殖，0.1~1.0 mmol·L-1时诱导Dami细胞向成熟巨核细胞分化。