小儿围手术期外周血清中总超氧化物歧化酶活力和丙二醛浓度的变化

摘要

The serum T-SOD and the MDA of the peripheral blood of 31 children(specimens obtained at half an hour before and after the operation,the first,the fourth,and the seventh day after operation)were measured seperately with the Xanthine Oxidase method and the improved Japanese method.The patients were divided into major,median and minor operation groups according to the degree of operative trauma,and 15 normal children as controls.The results showed:1.Preoperation:T-SOD was obviously lower and MDA was obviously higher in the major operation group(P<0.05).2.Postoperation:in the major or the median operation groups,the T-SOD became low and the lowest on the fourth day,or the MDA became high and the highest on the fourth day after operation.However,on the seventh day,these records tend to be normal(P<0.05),but no alteration in minor operations.3.The more extensive the operative injury,the faster the T-SOD dropped,or the MDA raised,and the longer the alterations lasted.4.The factors affecting TSOD were:the operative trauma,the method of anesthesia and the age of the child in the sequence of their importance.%用黄嘌呤氧化酶法和改良的八木国夫法分组检测31例患儿(围术期)、15例正常儿(术前对照)外周血清中总超氧化物歧化酶(T-SOE))活力和丙二醛(MDA)浓度.结果:①术前大手术组T-SOD活力低;MDA浓度高.②术后大、中手术组T-SOD活力降低,第4天最低;MDA浓度上升,第4天最高.二者第7天均有恢复趋势.小手术组无变化.③手术创伤大,术后T-SOD活力下降快;MDA浓度上升也快,且二者变化持续时间长.④对术后T-SOD活力的影响依次为手术创伤、麻醉方式及年龄.说明该结果可作为患儿围手术期治疗的参考依据.