AIM: To observe free radicals (MDA, NO) and iNOS of patients with severe acute respiratory syndrome (SARS) and to explore its significance. METHODS: MDA, NO2-/NO3- and iNOS were determined in SARS patients during the early, recovery and follow-up stage, front doctors and nurses (contact group) and health people (health control). RESULTS: The level of MDA during first stage was higher than that of recovery stage and the MDA level of recovery stage was higher than that of follow-up stage, contact group, and health control group (P<0.01). The content of NO2-/NO3- during early stage was higher than that of other groups, and the NO2-/NO3- contents of recovery stage, follow-up stage were higher than that of contact group and health control group (P<0.01), respectively. The mean of iNOS during early stage was highest than that of other stages (P<0.01) and the mean of recovery stage was higher than that of contact group (P<0.05), there were no difference in iNOS activity among any other groups (P>0.05). CONCLUSION: The pathological injury in pathogenesis of SARS is related to free radicals.%目的:监测SARS患者血清丙二醛(MDA)和一氧化氮(NO)、诱导型一氧化氮合酶(iNOS)浓度并探讨其意义.方法:采用硫代巴比妥酸比色法测定MDA、硝酸还原酶法测定NO、酶联免疫吸附法定量测定iNOS,检测早期、恢复期SARS患者以及出院后SARS随访者,一线未患SARS医护人员,健康体检者血清中MDA、NO和iNOS含量.结果:SARS早期血清MDA含量显著高于恢复期(P<0.01),恢复期均高于出院后随访时和一线医护人员与体检者(P<0.01).SARS早期组NO水平高于其他各组(P<0.05),恢复期组、随访组均与一线医护人员组和健康对照组有显著差异(P<0.01).SARS早期iNOS含量高于其他各期(P<0.01),恢复期与一线医护人员有显著差异(P<0.05),其余组间两两比较均无显著差异(P>0.05).结论:SARS的发病机制中病理损伤与自由基有关.
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