一氧化氮／诱导型一氧化氮合酶在动脉粥样硬化过程中的作用

摘要

AIM:To explore the dynamic changes of nitric oxide and inducible nitric oxide synthase during the process of atherosclerosis, and to analyze their influence on the formation of atherosclerosis .METHODS: SD rats (n=60) were randomly divided into control group and atherosclerosis group (30 rats in each group).Atherosclerosis model was induced by feeding high-fat diet and vitamin D 3 .The values of blood biochemical were analyzed enzymatically using bioMérieux kit.The concentration of serum nitric oxideing was detected by a colorimetric method .The success of athero-sclerosis modeling was determined by pathological examination .The protein expression of inducible nitric oxide synthase in atherosclerotic plaque was detected by the method of immunohistochemistry .RESULTS: The atherosclerosis model was successfully established in 90 d.The concentration of serum nitric oxide gradually decreased in atherosclerosis group , and a significant difference among groups was observed .Atherosclerosis index was positively correlated with calcium ion , and negatively correlated with nitric oxide .The protein expression of inducible nitric oxide synthase in the atherosclerotic plaque after 90 d was found.CONCLUSION:The protein expression of inducible nitric oxide synthase in the plaque area of aorta increases and the concentration of serum nitric oxide decreases with the process of atherosclerosis .The anti-atherosclerosis role of nitric oxide is gradually decreased .%目的：探讨一氧化氮（NO）/诱导型一氧化氮合酶（iNOS）在动脉粥样硬化（atherosclerosis，AS）过程中的动态变化，分析其对动脉粥样硬化形成过程的影响。方法：将60只SD大鼠随机分成2组：对照组及AS组，每组30只。 AS组采用维生素D3腹腔注射联合高脂饲料饲养的方法构建动脉粥样硬化模型。用相关生化方法检测血清各项生化指标：总胆固醇、甘油三酯、高密度脂蛋白胆固醇、低密度脂蛋白胆固醇、空腹血糖和钙离子，比色法检测血清NO浓度，并对主动脉行HE染色，免疫组化技术检测iNOS蛋白表达，将所得数据进行统计分析，用简单线性相关分析NO与钙离子及动脉粥样硬化指数的相关性。结果：90 d后成功构建了主动脉中膜钙化型动脉粥样硬化模型。血清NO浓度在动脉粥样硬化过程中逐步下降，各组间差异均有统计学意义（均P＜0．05）。动脉粥样硬化过程中动脉粥样硬化指数与钙离子呈正相关，与NO呈负相关。在90 d的AS组粥样斑块区免疫组化技术检测到iNOS蛋白表达。结论：在动脉粥样硬化形成过程中，主动脉粥样斑块区iNOS蛋白高表达，但血清NO浓度逐渐降低，NO抗动脉粥样硬化作用减弱。