柯萨奇病毒 B3感染 HeLa 细胞后 Bax 和 Bim 的表达

摘要

目的：观察HeLa细胞感染柯萨奇病毒B3（CVB3）后Bax和Bim mRNA和蛋白的表达。方法：将HeLa细胞分为未予以CVB3感染的对照组和予以CVB3感染的病毒组，取3 h、6 h、9 h、12 h和24 h 的细胞，采用RT-PCR和Western blotting分别检测Bax和Bim mRNA和蛋白的表达。结果：Bax和Bim mRNA在对照组和病毒组HeLa细胞中均有表达，在病毒组和对照组中Bax和Bim mRNA的表达在各自组内均无显著差异（P＞0.05），但在24 h Bax和Bim在病毒组mRNA的表达低于对照组（P＜0.05）。 Bax和Bim蛋白表达在对照组中各时点均有表达，但无显著差异（P＞0.05）。病毒组Bax蛋白在24 h表达下降，差异有统计学意义（P＜0.05），且病毒组表达明显低于对照组（P＜0.05）；Bim蛋白的表达随感染时间延长而下降（P＜0.05），9 h、12 h和24 h表达病毒组低于对照组（P＜0.05）。结论：Bax和Bim在CVB3感染诱导的HeLa细胞凋亡早期发挥作用。%AIM:To observe the expression of Bax and Bim at mRNA and protein levels in coxsackievirus B 3 (CVB3)-infected HeLa cells.METHODS:HeLa cells were divided into control (non-CVB3 infection) group and CVB3 infection group.The expression of Bim and Bax at mRNA and protein levels was determined by reverse transcriptase poly -merase chain reaction (RT-PCR) and Western blotting analysis at 3 h, 6 h, 9 h, 12 h and 24 h postinfection.RE-SULTS:The mRNA expression of Bax and Bim in control group was not significantly different among different time points , so was that in CVB3 infection group (all P>0.05).However, compared with control group, the mRNA expression of Bax and Bim in CVB3 infection groups was decreased at 24 h postinfection (all P<0.05).The protein expression of Bax and Bim in control group was not significantly different among different time points (P>0.05).However, in CVB3 infection group, the protein expression of Bax was lower at 24 h postinfection than that at 3 h (P<0.05).Meanwhile, compared with control group, the protein expression of Bax in CVB3 infection group was decreased at 24 h postinfection (P<0.05). The protein expression of Bim in CVB3 infection groups was decreased along with the time postinfection , and was decreased at 9 h, 12 h and 24 h as compared with control group (P<0.05).CONCLUSION:The proteins of Bax and Bim play an important role in CVB3-induced apoptosis of HeLa cells.