自拟补肾活血颗粒对去卵巢骨质疏松症大鼠的治疗作用

摘要

AIM: To observe the therapeutic effect of modified Bushen Huoxue granules (MBHG) on ovariec-tomized osteoporosis rats.METHODS: Female SD rats were randomly given sham operation (n =10) and ovariectomy, and then the model rats were further randomly divided into model group, MBHG treatment groups at doses of 200 mg/kg, 100 mg/kg and 50 mg/kg respectively, and positive control (estradiol valerate) group, with 10 rats in each group by intra-gastric administration for 12 weeks.The morphology, area, thickness, spacing and area percentage of trabecular bone in the rats were observed.The serum levels of calcium (Ca), phosphorus (P) and alkaline phosphatase (ALP) were mea-sured by automatic analyzer.Bone mineral density (BMD) was analyzed.Serum estradiol (E2 ), osteocalcin (BGP), os-teoprotegerin (OPG), receptor activator of nuclear factor-κB (RANK) and receptor activator of nuclear factor κB ligand (RANKL) levels were detected by ELISA.RESULTS: Compared with model group, trabecular bone significantly widened in all treatment groups with large number, and net-like structure restored partially.The thickness, area and area percenta-ges of trabecular bone in treatments groups were higher than those in model group,and trabecular spacing was less than that in model group (P <0.05).The serum Ca, P, E2 and OPG, and femoral BMD were significantly higher in treatment groups than those in model group, and the levels of ALP, BGP, RANK and RANKL were significantly lower than those in model group (P <0.01).CONCLUSION: MBHG has a significant therapeutic effect on ovariectomized osteoporosis rats. The mechanism may be related to the regulation of OPG and inhibition of RANKL secretion.%目的：观察自拟补肾活血颗粒（MBHG）对去卵巢大鼠骨质疏松症的治疗作用。方法：雌性 SD 大鼠，随机取10只作为假手术组（sham 组），其余大鼠制备去卵巢骨质疏松症，随机分为：模型（model）组；补肾活血颗粒高（200 mg／kg）、中（100 mg／kg）、低（50 mg／kg）剂量组；阳性对照组（戊酸雌二醇组）。每组10只，连续灌胃给药12周。观察大鼠骨小梁形态，测量骨小梁的面积、厚度、间距及面积百分数，全自动分析仪检测血清中钙、磷和碱性磷酸酶（ALP）含量，测量大鼠骨密度（BMD），ELISA 法检测血清雌激素（E2）、骨钙素（BGP）、骨保护素（OPG）、核因子κB 受体活化因子（RANK）和核因子κB 受体活化因子配体（RANKL）水平。结果：与模型组比较，各给药组骨小梁显著变宽，数目增多，网状结构有部分恢复。各给药组骨小梁厚度、骨小梁面积和骨小梁面积百分数均大于模型组，骨小梁间距均小于模型组（P ＜0．05）。各给药组血清中钙、磷、E2、OPG 以及股骨 BMD 水平均显著高于模型组，ALP、BGP、RANK 和 RANKL 水平均显著低于模型组（P ＜0．01）。结论：自拟补肾活血颗粒对去卵巢大鼠骨质疏松症有显著的治疗作用。作用机制可能与上调 OPG、抑制 RANKL 分泌有关。