倍他司汀对豚鼠单侧迷路破坏后前庭内侧核组胺H3受体表达的影响

摘要

目的 观察倍他司汀(betahistine)对单侧迷路毁损后的豚鼠前庭内侧核(medial vestibular nucleus)组胺H3受体表达的影响.方法 选择听性脑干反应(ABR)阈值小于40 dBSPL的健康黑色赤目纯种豚鼠56只,采用随机数字表法分为3组:假手术组(8只),单侧迷路破坏组(24只),单侧迷路破坏+倍他司汀组(24只).单侧迷路破坏组和单侧迷路破坏+倍他司汀组均损毁左侧迷路,假手术组手术打开听泡但保持迷路完好.单侧迷路破坏+倍他司汀组损毁迷路后给予倍他司汀2.17 mg/kg腹腔注射,每天1次,连续给药至处死当天;另外两组腹腔注射等量生理盐水.破坏单侧迷路的两组在不同观察时间点(术后1、3、7d)各取8只豚鼠通过免疫组织化学染色观察双侧前庭内侧核组胺H3受体的表达变化.采用SPSS 16.0软件对实验数据进行统计分析.结果 单侧迷路破坏组术后1d及3d,受损侧前庭内侧核组胺H3受体表达增加,与假手术组相比,差异具有统计学意义(P＜0.05),术后7d则恢复至正常水平,与假手术组相比差异无统计学意义(P＞0.05);未损侧前庭内侧核组胺H3受体表达强度各观察时间点与假手术组相比,差异无统计学意义(P值均＞0.05).单侧迷路破坏+倍他司汀组受损侧前庭内侧核组胺H3受体术后1d及3d较同期单侧迷路破坏组表达减弱(4.25 ±0.71、3.50 ±0.92 vs 5.75 ±0.71、5.50 ±0.93,P值均＜0.05);术后3d、7d与假手术组相比差异无统计学意义(P值均＞0.05).结论 豚鼠单侧迷路破坏后同侧前庭内侧核中组胺H3受体表达上调,倍他司汀可促进H3受体表达恢复至正常水平.%Objective To observe the influence of betahistine on the expression of histamine H3 receptor in the medial vestibular nucleus (MVN) following unilateral labyrinthectomy (UL).Methods Fifty-six healthy guinea pigs were randomly divided into three groups:the sham-operated group (group Ⅰ),the UL group [group Ⅱ,and UL + betahistine (BET) group (group Ⅲ)],BET was intraperitoneally injection at 2.17 mg · kg-1 · d-1 for 7 days.The expression of histamine H3 receptor was analyzed by immunohistochemistry at 1 day,3 days and 7 days after UL.Results H3 receptors were presented in the MVN and the expression of histamine H3 receptor were increased significantly in the ipsilateral MVN at 1 and 3 days after UL(P ＜0.05),the change turned into the normal value at 7 days(P ＞0.05).In the UL + BET group,the intensity of histamine H3 receptor was lower than that in the UL at 1 day and 3 days(4.25 ± 0.71,3.50 ± 0.92 vs 5.75 ± 0.71,5.50 ± 0.93,P ＜ 0.05).However,the changes turned into the normal values at 3 and 7 days (P ＞ 0.05).Conclusions The early stage of the vestibular compensation process may be associated with the change of H3 receptor expression in MVN.In the UL + BET group the histamine H3 receptor recovered quickly.