破骨细胞对IGF-1诱导成骨细胞骨同化的促进作用

摘要

目的 探讨破骨细胞(osteoclast,OC)存在时IGF-1对成骨细胞(osteoblast,OB)活性的影响.方法 体外培养MC3T3小鼠成骨细胞及RANKL诱导分化成熟的破骨细胞.以10 ng/ mL的rhIGF-1直接干预单独或与破骨细胞共育的成骨细胞,并设空白对照组,培养12 h,检测成骨细胞的增殖、凋亡及碱性磷酸酶活性;培养8d后Von Kossa钙化染色法观察矿化结节的形成.结果 成骨细胞、破骨细胞共培养时,rhIGF-1作用12h能够明显促进成骨细胞增殖(P＜0.05),抑制成骨细胞的凋亡(P＜0.05),使细胞内ALP活性升高(P＜0.05),8d时钙化结节的个数及面积百分比升高(P＜0.05);与单独培养的成骨细胞组有显著性差异.结论 破骨细胞可明显促进IGF-1所诱导的成骨细胞骨同化作用.%Objective To investigate the effect of IGF-1 on the activity of osteoblasts in the presence of osteoclasts. Methods Mice MC3T3 osteoblasts and mature differentiated osteoclasts induced by RANKL were cultured in vitro. Osteoblasts were cultured in the presence of 10 ng/mL rhIGF-1 alone or co-cultured with 10 ng/mL of rhIGF-1 and osteoclasts. Blank control group was established. After 12-hour culture, the proliferation and apoptosis of osteoblasts and the activity of alkaline phosphatase ( ALP ) were tested. After 8-day culture, the formation of mineralization nodes was observed using von Kossa staining. Results When osteoblasts were co-cultured with osteoclasts, rhIGF-1 could significantly promote the proliferation of osteoblasts ( P < 0. 05 ), inhibit the apoptosis of osteoblasts ( P < 0. 05 ) , and up-regulate the activity of ALP ( P <0. 05 ) after 12-hour culture. The number of mineralization nodes and area ratio increased after 8-day culture ( P < 0. 05 ), and the difference was significant when compared with that in mono-cultured group ( Prn<0. 05 ). Conclusion Osteoclasts can significantly promote the osteoblast bone formation induced by IGFrn-1.