不同频率脉冲电磁场对骨保护素基因敲除小鼠骨代谢指标影响

摘要

目的 采用不同频率的脉冲电磁场干预骨保护素基因敲除小鼠骨质疏松模型,探讨脉冲电磁场治疗骨质疏松的最适治疗频率.方法 8周龄雌雄各半骨保护素基因敲除小鼠30只,随机分为A、B、C 3组,每组10只,A组为空白对照组,不进行干预;B组小鼠每天在强度为4 mT,频率为8 HZ的磁场中照射治疗20 min,共30 d;C组小鼠每天在强度为4 mT,频率为32 HZ的磁场中照射治疗20 min,共30 d.各组小鼠在治疗前及治疗30 d后用酶联免疫法(ELISA)法检测抗酒石酸酸性磷酸酶5b及血清骨钙素值.结果 8 HZ、32 HZ两治疗组在治疗后抗酒石酸酸性磷酸酶5b,血清骨钙素含量与A对照组之间比较差异有统计学意义(P＜0.05),8 HZ、32 HZ两治疗组之间治疗前与治疗后所测三种指标比较差异无统计学意义(P>0.05).结论 脉冲电磁场对骨保护素基因敲除小鼠骨质疏松有肯定治疗效果,应用频率为8～32 HZ的磁场治疗能产生相同的治疗效果.%Objective To explore the optimal frequency of pulsed electromagnetic fields for the treatment of osteoporosis, by using different frequencies of pulsed electromagnetic fields to treat osteoprotegerin ( OPG ) knock-out mouse model Methods Fifteen male and fifteen female 8-week OPG knock-out mice were randomly divided into 3 groups: Group A, Group B, and Group C. Each group had 10 mice. Group A was blank control group with no intervention. Mice in Group B received PEMFs treatment at 8 Hz frequency with 4.0 mT intensity, 20 min per day, for 30 days. Mice in Group C received PEMFs treatment at 32 Hz frequency with 4.0 mT intensity, 20 min per day, for 30 days. Tartrate-resistant acid phosphatase 5b and serum bone 7 carboxyglutamic acid of all rats in each group were measured using ELISA before the treatment and 30 days after the treatment. Results The concentrations of tartrate-resistant acid phosphatase 5b and serum bone 7 carboxyglutamic acid in Group B and C after the treatment were significantly different with those in Group A ( P < 0. 05 ). Whereas the bone metabolism markers in Group B before or after the treatment had no significant difference compared with those in Group C ( P > 0. 05 ). Conclusion The treatment of pulsed electromagnetic fields for osteoprotegerin gene knock-out mice is really effective. Both the frequency of 8 HZ and 32 HZ can produce the same therapeutic effect.