不同浓度 hTNFα对软骨细胞及其基质相关基因表达的影响

摘要

Objective To investigate the effect of tumor necrosis factor ( TNFα) on the secretion of matrix protein and mRNA expression of related genes in cartilage cells.Methods Mouse chondrocytes were cultured in vitro and were additioned with 25, 50, and 100 ng/ml of hTNFα.No hTNFαcultures were as control.mRNA expression of related genes in chondrocytes was detected using RT-qPCR.Results hTNFαreduced type II collagen secretion in cartilage cells and stimulated SOX9 gene expression that stimulates pro-II collagen secretion.It increased gene expression of MMP-9, MMP-13, ADAMTS-4, and ADAMTS-5 in the matrix.It also increased inflammation factors IL-1αand TGFβ, and increased apoptotic gene expression of BAX and caspase 3. Conclusion hTNFαaffects the expression of matrix-related genes in cartilage cells by different ways, thus stimulating the degradation of articular cartilage.%目的：探讨软骨细胞在肿瘤坏死因子（ TNFα）作用下对其分泌基质蛋白及相关基因mRNA表达的影响。方法体外培养的小鼠软骨细胞用25，50，100 ng／ml的hTNFα干预，不加入TNFα作为对照，通过RT-qPCR检测hTNFα对软骨细胞分泌基质蛋白及相关基因mRNA表达的影响。结果 hTNFα能降低软骨细胞分泌的Ⅱ型胶原及刺激促Ⅱ型胶原分泌的SOX9基因的表达；对基质可提高基质酶MMP-9，MMP-13，ADAMTS-4和ADAMTS-5基因的表达，提高炎性因子IL-1α，TGFβ，并增加凋亡基因BAX， caspase3的表达。结论 hTNFα通过不同途径影响软骨细胞及基质相关基因的表达，从而促进了关节软骨的降解。