重组成骨蛋白－1对磨损微粒诱导成骨细胞炎性因子的影响

摘要

目的：探讨重组成骨蛋白－1（rOP－1）对钛－6铝－4钒微粒（Ti－6Al－4V）诱导成骨细胞中炎性因子如肿瘤坏死因子－α（ TNF－α）、白介素－1β（ IL－1β）和白介素－6（ IL－6）的影响。方法将成骨细胞分为正常对照组、 rOP－1组、 Ti－6Al－4V组， Ti－6Al－4V＋rOP－1组，采用Elisa法检测各组细胞培养72 h后TNF－α、IL－1β和IL－6含量的变化。结果培养72 h后，与Ti－6Al－4V组比较， Ti－6Al－4V＋rOP－1组TNF－α、 IL－1β和IL－6含量下降，抑制率分别为25．67％、7．78％和21．86％，差异有统计学意义（均P＜0．05）。结论 rOP－1可抑制Ti－6Al－4V诱导成骨细胞炎性因子TNF－α、 IL－1β和IL－6，为为防治关节假体无菌性松动提供新的方法。%Objective To investigated recombinant osteogenic protein 1 affects of TNF-α, IL-1β, and IL-6 on Ti-6Al-4V-induced osteoblast.Methods Osteoblasts were divided into 4 groups: control group, rOP-1 group, Ti-6Al-4V group and Ti-6Al-4V with rOP-1 group, Elisa was used to assay effects of inflammatory cytokines after 72 h incubation with Ti-6Al-4V (rOP-1).Results Compared with the osteoblasts with particle group, after 72 h-incubation, the TNF-α, IL-1β, and IL-6 of osteoblasts in Ti-6Al-4V with rOP-1 group was significantly decreased , inhibition 25.67%, 7.78%, and 21.86%, respectively (P<0.05).Conclusion rOP-1 decreased level of TNF-α, IL-1βand IL-6 on osteoblast and could be therapeutic candidate for the prevention of artificial joint aseptic loosening .