甘蓝型油菜BnGPAT9基因表达模式及其苗期非生物胁迫表达分析

摘要

To understand glycerol-3-phosphate acyltransferase (GPAT;EC2.3.1.15) gene under abiotic stress,we cloned BnGPAT9 gene (GenBank:KX344511.1) of B.napus.Sequence analysis showed that the gene was 1 13 1bp long,encoding 376 amino acids.Homology analysis showed that BnGPAT9 protein was highly homologous to other GPAT9 proteins from different plants,and was highly conserved in the process of biological evolution.qRT-PCR results showed that BnGPAT9 expressed specially in 1-4 week-seeds after flowering and in the stem,flower,leaf,root and silique wall.Abiotic stress (at 5-6 leaf stage) results showed that the expression of BnGPAT9 was enhanced by PEG-drought and high temperature,but was inhibited by low temperature,6-BA,ABA and salt.Z pattern was found under waterlogging.The expression reached the lowest at 12h,and returned at 24h.%为揭示油菜BnGPAT9基因的功能及其在非生物胁迫中的表达变化,克隆甘蓝型油菜BnGPAT9基因(GenBank:KX344511.1)并分析表达模式.发现该基因全长1 131bp,编码376个氨基酸残基.序列比对及系统进化树分析表明BnGPAT9与其它植物的GPAT9有很大的相似性,在生物进化过程中具有很高的保守性.实时荧光定量PCR分析表明,BnGPAT9基因在甘蓝型油菜中的表达具有组织特异性,在开花后1～4周幼嫩的种子中表达较强,而在茎、花、叶、根和角果皮中表达较弱;5 ～6片真叶期盆栽苗的非生物胁迫表达分析显示,短期干旱和高温能够诱导和促进叶片BnGPAT9基因的表达;低温、6-BA、ABA和盐胁迫抑制其表达;水渍对BnGPAT9基因的表达影响呈“Z”字形,12h出现最低值,但24h后又开始回升.