Objective To observe the injury of nickel sulphate to the lens of Wistar rat and the protection of Qiguijiedu soup against the damage.Methods Wistar rats were randomly grouped,as Nickel sulphate group (the mice were administered nickel sulphate 15 mg/kg by subcutaneous injection),Qiguijiedu Soup group (the mice were intragastricly administered with the drug 1 ml/100 g.7 days later,the same solution was administered to the rats two times a day and nickel sulphate was subcutaneously injected one time a day simultaneously),Tiopronin Tablets group ( the mice were intragastricly administered with the drug 0.23 g/kg in the form of its solution.7 days later,the same solution was administered to the rats two times a day and nickel sulsphate was administered as it was in Qiguijiedu Soup group),and the blank group with each group of 15 rats.The process of injecting nickel sulphate lasted for 45 days.The LOCS Ⅲ System was used to evaluate the morphologic changes in the lens with the double-blind method.Finally,the rats were sacrificed and the lens were extracted and fixed with the Formalin.Atomic absorption photometer was used to detect the amount of nickel sulphate in the lens.All statistical analysis was performed with the statistics program SPSS (version 16.0).Results In P area of the lens,score in the nickel sulphate group was higher than that in Qiguijiedu Soup group Tioronin Tablet group and the blank group.The differences were significant ( F =35.28,P ＜ 0.05 ).In area N of the lens,scores in the Nickel Sulphate group were higher than that in Qiguijiedu Soup group,Tioronin Tablet group and blank group.The differences were significant ( F =78.64,P ＜ 0.05).Area A or C of the lens appeared with no obvious opacity,and no significant differences were found ( F =0.173,P =0.914 ; F =0.364,P =0.779).Area A plus P of the lens,scores in the nickel sulphate group were higher than that in Qiguijiedu Soup group,in Tioronin Tablet group,or in blank group,and the significant differences were found among the four groups ( F =30.24,P ＜ 0.05 ).When the lens were analyzed as a whole,score in Nickel sulphate group was higher than that in Qiguijiedu Soup group,in Tioronin Tablet group,or in the blank group,and the differences were significant ( F =38.14,P ＜0.05 ).While the amount of nickel in Nickel Sulphate group was higher than that in Control group ( Z =- 3.100,P ＜ 0.05 ),which was lower than in Qiguijiedu Soup group ( Z =- 3.920,P ＜ 0.05 ).There was no difference in the amount of nickel between the Tioronin group and Qiguijiedu Soup group( F =0.035,P ＞0.05).Conclusion Nickel sulphate would injure the lens of Wistar rats and Qiguijiedu soup could protect the lens from the injury.%目的 探讨硫酸镍对Wistar大鼠晶状体的损伤情况及芪归解毒汤对其保护作用.方法 选用出生7～14d的Wistar大鼠,随机分为芪归解毒汤预防组、障眼明预防组、硫酸镍组、空白对照组,每组15只.芪归解毒汤预防组:芪归解毒汤按1 ml/100 g给药灌胃7d后,再同时芪归解毒汤灌胃每天2次和硫酸镍溶液皮下注射每天1次,连续45 d；障眼明预防组:障眼明溶液按0.23 g/kg给药灌胃7d后,再同时用障眼明溶液灌胃每天2次和硫酸镍溶液皮下注射每天1次,连续45d；硫酸镍对照组:用2 mg/ml的硫酸镍溶液按15 mg/kg的剂量连续皮下注射45 d；空白组:Wistar大鼠不做任何处理,同等条件下正常饲养45 d.45d后双盲法在裂隙灯显微镜下参照LOCSⅢ评价Wistar大鼠晶状体的浑浊程度.最后处死大鼠,取下完整晶状体,置于10％福尔马林溶液中4℃保存,用原子吸收分光光度计测定镍含量.用SPSS 16.0软件进行单因素方差分析或秩和检验.结果 晶状体浑浊度观察:在晶状体P区域中,硫酸镍组晶状体浑浊指数得分高于芪归解毒汤预防组、障眼明预防组及对照组,差异有统计学意义(F=35.28,P＜0.05)；在晶状体N区域中,硫酸镍组晶状体浑浊指数得分高于芪归解毒汤预防组、障眼明预防组及空白对照组,差异有统计学意义(F=78.64,P＜0.05).在晶状体A、C两区域,晶状体浑浊指数得分各组之间差异无统计学意义(F=0.173,P=0.914；F=0.364,P=0.779)；在晶状体A+P区域上,硫酸镍组晶状体浑浊指数得分高于芪归解毒汤预防组、障眼明预防组及空白对照组,差异有统计学意义(F=30.24,P＜0.05).晶状体A+C+N+P4个区域浑浊指数得分总和分析,硫酸镍组晶状体浑浊指数得分高于芪归解毒汤预防组、障眼明预防组及空白对照组,差异均有统计学意义(F =38.14,P＜0.05)；硫酸镍含量测定:硫酸镍组高于空白对照组(Z=-3.100,P＜0.05)；芪归解毒汤预防组低于硫酸镍组(Z=-3.920,P＜0.05)；障眼明预防组比较,差异无统计学意义(F=0.035,P＞0.05).结论 硫酸镍皮下注射能对Wistar大鼠晶状体造成一定的损伤,芪归解毒汤能有效减轻硫酸镍对Wistar大鼠晶状体的损伤.
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