鲨肝肽对小鼠免疫性肝损伤的保护作用及免疫调节作用

摘要

Objectives:To explore the effect of Shahepatide,an a ctive polypeptide extracted from shark liver,on secretion of IFN-γ and TNP-β from human periperal blood mononuclear cells (PBMC)and to explore its liver-pr otective effect in mice with immune liver injury.Methods:Shahepati de was added to PBMC and cultured.The content of IFN-γ and TNF-β in the cult ure supernatant were measured.Mice model of immune liver injury was created by endotoxin attack and their blood AST and ALT level were determined.Resul ts:Shahepatide can induce secretion of IFN-γ from PBMC(P<0.01),inhib it production of TNF-β from PBMC,can significantly reduce the abmomal elevatio n of serum ALT and AST in mice with immune liver injury and obviously reduce the ir pathological liver injury.Conclusions:Shaheptide possesses an e xcellent prospect in treatment of hepatitis B,liver cirrhosis,and chronic hepati tis,as well as in modulation of immunologic functions.%目的：探讨从海洋生物鲨鱼肝纯化获得的一种活性多 肽（ 以下称鲨肝肽）对人外周血单核细胞（PBMC）分泌IFN-γ及TNF-β的影响，以及对小鼠免 疫性肝损伤的保护作用。方法：分离PBMC，加入鲨肝肽，检测培养上 清中IFN-γ，TNF-β的量，另取健康小鼠，以内毒素攻击制造免疫性肝损伤模型，定时测 定血中AST，ALT的量。结果：鲨肝肽能有效诱导PBMC分泌IFN-γ； 抑制PBMC产生TNF-β；显著降低免疫性肝炎小鼠血清转氨酶含量的异常升高，肝脏病理损 伤明显减轻。结论：鲨肝肽在乙型肝炎、迁慢性肝炎、肝硬化等肝病 的治疗及调节机体免疫功能方面具有良好的应用前景。