目的:采用同时测定犬血浆中4种银杏内酯(银杏内酯A,B,C和白果内酯)和3种黄酮(槲皮素、山柰酚、异鼠李素)的高效液相色谱-串联质谱(HPLC-MS/MS)分析方法,研究了银杏叶酊剂的相对生物利用度.方法:相对生物利用度试验采用随机分组,两周期交叉设计.犬血浆样品经过酸化处理后,采用乙酸乙酯萃取.运用所建立的HPLC-MS/MS方法测定其血药浓度.结果:银杏叶酊剂和参比制剂(银杏叶提取物滴剂和银杏叶片剂)相比较,7个有效成分的吸收差异较大,其中白果内酯受试制剂吸收差于参比制剂；其他成分受试制剂吸收好于或者与参比制剂相当.结论:所采用的HPLC-MS/MS方法快速、灵敏、准确,受试制剂和参比制剂中有效成分的相对生物利用度存在一定差异.%Objective: To establish a high-performance liquid chromatography-tandem mass spectrometry ( HPLC-MS/MS) method for determination of terpenoids ( ginkgolides A, B and C; bilobalide) and flavonoids (quercetin, kaemferol and isorhamnetin) in dog plasma, and investigate the relative bioavailability of the tincture extract of Ginkgo biloba leaves. Methods: Three preparations were administrated to male beagle dogs according to a randomized cross design. Dog plasma samples were extracted with EtOAc after adding HCl (6 mol·L-1). Then, the concentrations of 7 compounds in dog plasma were determined by HPLC-MS/MS. Results: Compare with reference preparations (extract drops and extract tablets of Ginkgo biloba leaves) , the 7 active compounds in tincture extract of Ginkgo biloba leaves were absorbed better or equal except biloblide. Conclusion: The HPLC-MS/MS method is rapid, sensitive and reliable. The relative bioavailability of the test preparations shows the difference from the reference preparations.
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