冰片和麝香酮对神经生长因子血脑屏障通透性的影响研究

摘要

Objective To explore the influences of borneol and muscone on blood-brain barrier permeability of nerve growth factors.Methods Thirty-two Wistar rats aged 7 days old were divided into the muscone group, borneol group, control group and borneol+muscone group, with eight rats in each group.The levels of nerve growth factors passing the blood-brain barrier and neural function behavior scores of the patients in the four groups were analyzed and compared . Results The levels of nerve growth factors in Evans blue, brain tissue and blood of rats in the borneol+muscone group were higher than those in the other three groups .Neural function behavior score of rats in the borneol+muscone group was lower than that in the other three groups(P<0.01).There were no significant differences in the content of Evans blue, level of nerve growth factors and neural functional behavior scores between the borneol group and the muscone group (P>0.05).The content of Evans blue, level of nerve growth factors, neural function behavior scores in the borneol group and the muscone group were higher than those in the control group(P<0.01).Conclusion Muscone and borneol can raise the permeability of blood-brain barrier and get nerve growth factors across the blood-brain barrier better.%目的研究冰片和麝香酮对神经生长因子血脑屏障通透性的影响。方法选取32只出生7 d Wistar大鼠进行研究，根据用药的不同分为麝香酮组、冰片组、对照组、冰片＋麝香酮组，每组8只，分析比较四组大鼠神经因子透过血脑屏障的含量及神经功能行为评分。结果冰片＋麝香酮组的伊文思蓝含量、脑组织及血液中神经生长因子含量均高于其他三组，神经功能行为评分均低于其他三组（ P＜0．01）。冰片组与麝香酮组伊文思蓝含量、脑组织及血液中神经生长因子含量、神经功能行为评分比较差异无统计学意义（P＞0．05），但两组伊文思蓝含量、脑组织及血液中神经生长因子含量、神经功能行为评分均高于对照组（ P＜0．01）。结论应用麝香酮和冰片可以提高血脑屏障的通透性，神经生长因子进入中枢神经系统的量更多，利用率更高。