肝癌患者中 microRNA－378与 FOXO1基因表达的相关关系研究

摘要

Objective To study the expressions of miR-378 and FOXO1 in hepatocellular carcinoma ( HCC) and the correlation of the expressions between miR-378 and FOXO1 genes.Methods Matched normal and cancerous tissues from 8 patients with HCC were examined for miR-378 and FOXO1 expressions using real-time quantitative PCR.Independent-Samples T test was used to test the significance of the means of the expressions of miR-378 and FOXO1 between normal and cancerous tissues .The correlation of the expressions between miR-378 and FOXO1 in cancerous tissues was detected by bivariate correlation analysis .Results The expressions of miR-378 and FOXO1 in HCC cancerous tissues were both significantly lower than those in adjacent tissues ( P=0.0106 and P=0.000623 ) . The expression of miR-378 was positively correlated with that of FOXO 1 ( r=0.541 , P=0.031 ) in cancerous tis-sues.Conclusion The expressions of miR-378 and FOXO1 in HCC tissues are significantly lower than those of the adjacent tissues .The abnormal expressions of miR-378 and FOXO1 and their interaction network may play a vital role in HCC development .%目的：研究microRNA－378a－3p（miRNA－378）与FOXO1基因在肝癌中的表达情况及两者表达的相关性。方法提取8例肝癌患者癌组织及其相对应癌旁组织标本的总RNA，采用实时荧光定量PCR分别检测miR－378和FOXO1基因的相对表达量，通过独立样本t检验比较癌及癌旁组织中miR－378、FOXO1基因表达的差异，并通过双变量相关分析探索癌组织中两者之间的相关关系。结果肝癌组织中miR－378和FOXO1基因的表达量均明显低于癌旁组织中的表达（ P＝0．0106和P＝0．000623），两者相对表达量在肝癌组织中呈正相关关系（ r＝0．541，P＝0．031）。结论肝癌组织中miR－378与FOXO1基因的表达较癌旁组织明显降低，miR－378与FOXO1基因的异常表达及其相互作用网络在肝癌发生发展中可能具有重要的意义。