长链非编码RNA MEG3在胶质瘤细胞U87中生物功能的研究

摘要

目的 探讨长链非编码RNA母系印记基因3(MEG3)在神经胶质瘤中的表达及对U87胶质瘤细胞生物学功能的影响.方法 采用实时荧光定量聚合酶链反应(qRT-PCR)方法检测116例新鲜神经胶质瘤组织标本及癌周正常组织标本中长链非编码RNA MEG3的表达;MEG3过表达后,分别采用四氮唑蓝盐化合物(MTS)法、流式细胞仪和Transwell实验检测其对神经胶质瘤细胞增殖、凋亡和迁移的影响.结果 MEG3在神经胶质瘤组织中的表达显著低于正常组织(4.85±0.56 vs 9.18±0.45;=1.67,P=0.0012 ＜0.05);过表达MEG3后胶质瘤细胞U87增殖能力显著降低,差异具有统计学意义(62.0％±3.8％ vs 84.0％±4.6％;t=1.54,P=0.0145 ＜0.05);过表达MEG3后,细胞凋亡能力明显下降,差异具有统计学意义(19.4％ ±3.2％ vs5.5％±1.2％;t=2.35,P=0.0013＜0.05);过表达MEG3后,U87细胞迁移能力显著下降,差异具有统计学意义(55.6％±5.8％vs 100％±0;t=1.48,P=0.0021 ＜0.05).结论 MEG3在神经胶质瘤中是低表达的,具有抑制细胞U87增殖和迁移,促进凋亡的作用,在神经胶质瘤的治疗中具有潜在的应用价值.%Objective The expression and biological functions of LncRNA maternally expressed gene 3 (MEG3) in glioma were investigated.Methods Real-time quantitative polymerase chain reaction (qRT-PCR) was used to detect lncRNA MEG3 expression of 116 cases of glioma and adjacent normal brain tissues.And MTS [3-(4,5-diethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-etrazolium,inner salt] assay,flow cytometry and Transwell assay were further used to analyze the proliferation,apoptosis,and migration of MEG3 overexpression.Results LncRNA MEG3 expression was significantly decreased in the glioma compared with the normal tissues (4.85 ±0.56 vs 9.18 ±0.45;t =1.67,P =0.0012 ＜0.05);overexpression of MEG3 inhibited the cell proliferation (62.0％ ±3.8％ vs 84.0％ ±4.6％;t =1.54,P =0.0145 ＜0.05),promoted the cell apoptosis (19.4％ ± ±3.2％ vs 5.5％ ± 1.2％;t =2.35,P =0.0013 ＜0.05) and inhibited the cell migration (55.6％ ± 5.8％ vs 100％ ± 0;t =1.48,P=0.0021 ＜0.05) in U87 human glioma cell line.Conclusion LncRNA MEG3 is lowly expressed and indicates the inhibition of proliferation and migration and promotion of apoptosis in glioma and implicates the potential application of MEG3 in glioma therapy.